TRAIL/TNFSF10 Antibody (N2B2)

Images

 
Flow Cytometry: TRAIL/TNFSF10 Antibody (N2B2) [NBP1-43272] - Analysis using the Biotin conjugate of NBP1-43272. Staining of TRAIL transfected cells with Rat IgG2 Isotype Control (NBP1-43321) (open) or 0.125 ug of ...read more
Flow Cytometry: TRAIL/TNFSF10 Antibody (N2B2) [NBP1-43272] - Staining of mouse TRAIL-transfected cells with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD253 (TRAIL) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, Func, IP
Clone
N2B2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates

TRAIL/TNFSF10 Antibody (N2B2) Summary

Immunogen
The immunogen for this antibody was Trail.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
TNFSF10
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunoprecipitation 1:10-1:500
Application Notes
The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for TRAIL/TNFSF10 Antibody (N2B2)

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAIL
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10

Background

The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3- NK1.1+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-43516

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Bioinformatics

Gene Symbol TNFSF10
Entrez