TRAIL R2/TNFRSF10B Antibody

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Western Blot: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Total protein from HL-60, HepG2, HCT-116 and human placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk ...read more
Immunocytochemistry/ Immunofluorescence: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Western Blot: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis using the Azide Free version of NB100-56618. Detection of 20 ug of whole cell lysates from HL60 cells with anti-TRAIL-R2 at 5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Formulation Size Price

TRAIL R2/TNFRSF10B Antibody Summary

Immunogen
Rabbit anti-DR5 polyclonal antibody was raised against a peptide corresponding to amino acids 388 to 407 (isoform 2) of human DR5 precursor (1,2). The same sequence is found in isoform 1 at amino acids 417-436.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNFRSF10B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 5 ug/ml
  • Flow Cytometry 1:20-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Flow Cytometry (Cell Surface): As reported in KOYAMA (2003) Immunocytochemistry/Immunofluorescence: As reported in Georgakis et al. (2005) Immunohistochemistry (paraffin): As reported in Younes et al. (2006). Use in Immunohistochemistry reported in scientific literature (PMID 27740879).
Positive Control
Jurkat Lysate (NB800-PC2)
HL-60 Lysate (NB800-PC3)
TRAIL R2/TNFRSF10B Lysate (NBL1-17142)
Publications
Read Publications using
NB100-56618 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TRAIL R2/TNFRSF10B Antibody

  • CD262 antigen
  • CD262
  • death domain containing receptor for TRAIL/Apo-2L
  • Death receptor 5
  • DR5
  • DR5TRICK2B
  • Fas-like protein
  • KILLER/DR5
  • KILLERapoptosis inducing protein TRICK2A/2B
  • TNF-related apoptosis-inducing ligand receptor 2
  • TNFRSF10B
  • TRAIL R2
  • TRAIL receptor 2
  • TRAILR2
  • TRAIL-R2apoptosis inducing receptor TRAIL-R2
  • TRAILR2cytotoxic TRAIL receptor-2
  • TRICK2
  • TRICK2A
  • TRICKB
  • tumor necrosis factor receptor superfamily member 10B
  • tumor necrosis factor receptor superfamily, member 10b
  • tumor necrosis factor receptor-like protein ZTNFR9
  • ZTNFR9

Background

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRAIL R2/TNFRSF10B Antibody (NB100-56618)(37)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, Flow-CS, ICC/IF, IHC, IHC-P, WB.


Filter By Application
FLOW
(4)
Flow-CS
(3)
ICC/IF
(4)
IHC
(2)
IHC-P
(2)
WB
(10)
All Applications
Filter By Species
Human
(13)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NB100-56618 Applications Species
Shin GC, Kang HS, Lee AR, Kim KH Hepatitis B virus-triggered autophagy targets TNFRSF10B/death receptor 5 for degradation to limit TNFSF10/TRAIL response. Autophagy Oct 14 2016 [PMID: 27740879] (WB, IHC, Human) WB, IHC Human
Allen JE, Crowder R, El-Deiry WS. First-In-Class Small Molecule ONC201 Induces DR5 and Cell Death in Tumor but Not Normal Cells to Provide a Wide Therapeutic Index as an Anti-Cancer Agent. PLoS ONE. 2015 Nov 20 [PMID: 26580220] (FLOW, Human)

Details:
DR5 (TRAIL R2/TNFRSF10B) antibody was used for FLOW analysis of HCT116 (tumor) and HFF (normal) cells and the protocol implicated - fixation of cells in 4% paraformaldehyde-PBS for 20 minutes, use of primary at 1:250 dilution with ON incubation, detection using Alexa fluor 488 conjugated anti-rabbit secondary (Fig 2).
FLOW Human
Liu Y, Hawkins OE, Vilgelm AE et al. Combining an Aurora Kinase Inhibitor and a Death Receptor Ligand/Agonist Antibody Triggers Apoptosis in Melanoma Cells and Prevents Tumor Growth in Preclinical Mouse Models Clin. Cancer Res. 2015 Jul 07 [PMID: 26152738] (WB, Human)

Details:
TRAIL R2/TNFRSF10B (DR5) antibody was used for WB on lysates of human melanoma cell line Hs294T treated with mock siRNA or DR5-siRNAs (Figure 3D).
WB Human
Foster KA, Jane EP, Premkumar DR et al. NVP-BKM120 potentiates apoptosis in tumor necrosis factor-related apoptosis-inducing ligand-resistant glioma cell lines via upregulation of Noxa and death receptor 5 Int. J. Oncol. 2015 Jun 05 [PMID: 26044191] (ICC/IF, Human) ICC/IF Human
Talekar MK, Allen JE, Dicker DT, El-Deiry WS. ONC201 induces cell death in pediatric non-Hodgkin's lymphoma cells Cell Cycle. 2015 Jun 01 [PMID: 26030065] (FLOW)

Details:
DR5 antibody (Imgenex Img-120A) was used at 1:200 dilution for FLOW applictaion in experiments involving ONC201 induced cell death in cell lines namely Granta, UPN2, NCEB, Karpas299, BJAB, Daudi cell lines (Fig. S1A, B).
FLOW
Prabhu VV, Allen JE, Dicker DT, El-Deiry WS. Small molecule ONC201/TIC10 targets chemotherapy-resistant colorectal cancer stem-like cells in an Akt/Foxo3a/TRAIL-dependent manner Cancer Res. 2015 Feb 20 [PMID: 25712124] (FLOW, Human)

Details:
TRAIL R2/TNFRSF10B antibody was used for WB and FLOW applications in experiments involving colon cancer cell lines namely SW480, DLD1, and HCT116 cells and HCT116 cells overexpressing myristoylated Akt. For FLOW, the cells were fixed in 4% paraformaldehyde, incubated with primary antibody at 1:100 dilution or with normal IgG control for ON or 2 hours at 4C, washed and incubated with secondary antibody which followed analysis on Elite ESP flow cytometer (Supplementary Figure S5). WB performed on cancer cells subjected or not to SiRNA knockdown of DR5 (Supplementary Figure S6 ).
FLOW Human
Xu J, Zhou JY, Xu Z et al. The role of Cullin3-mediated ubiquitination of the catalytic subunit of PP2A in TRAIL signaling Cell Cycle. 2015 Jan 01 [PMID: 25551360] (WB, Human)

Details:
TRAIL R2/TNFRSF10B (DR5) antibody used for WB on DISC-Flag immunoprecipitates from lysates of SUM159 cells (human breast cell line) that were treated with 100 ng/ml Flag-TRAIL, 1 mg/ml Enhancer and 2 mg/ml anti-Flag (Flag/M2) for 30 min at 37 C (Figure 3C).
WB Human
Chen Jun-Jie, Bozza William P, Di Xu et al. H-Ras regulation of TRAIL death receptor mediated apoptosis. Oncotarget. 2014 Jul 15 [PMID: 25026275] (Human) Human
Qiu F, Hu M, Tang B, Liu X et al. Annexin V-TRAIL fusion protein is a more sensitive and potent apoptotic inducer for cancer therapy. Sci Rep 2013 Dec 20 [PMID: 24356445] (WB, Human) WB Human
Xu J, Xu Z, Zhou JY et al. Regulation of the Src-PP2A Interaction in Tumor Necrosis Factor (TNF)-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis. J Biol Chem 2013 Nov 15 [PMID: 24100030] (WB, Human)

Details:
BT549 human breast carcinoma cells (lipid raft and non-raft membrane fractions), Fig 5A, 5B;DR5 was more highly detected in Non-raft than raft membrane fractions.
WB Human
Show All 37 Publications.

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Positive Control Lysate(s)

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Bioinformatics

Gene Symbol TNFRSF10B
Entrez