Western Blot: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Total protein from HL-60, HepG2, HCT-116 and human placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk ...read more
Immunocytochemistry/ Immunofluorescence: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis of a formalin fixed paraffin-embedded (FFPE) human brain cerebellum using 1:200 conc. of TRAIL R2/TNFRSF10B antibody on a Bond Rx ...read more
Western Blot: TRAIL R2/TNFRSF10B Antibody [NB100-56618] - Analysis using the Azide Free version of NB100-56618. Detection of 20 ug of whole cell lysates from HL60 cells with anti-TRAIL-R2 at 5 ug/ml.
Rabbit anti-DR5 polyclonal antibody was raised against a peptide corresponding to amino acids 388 to 407 (isoform 2) of human DR5 precursor (1,2). The same sequence is found in isoform 1 at amino acids 417-436.
Protein G purified
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Flow Cytometry (Cell Surface): As reported in KOYAMA (2003) ICC/IF: As reported in Georgakis et al. (2005) IHC-P As reported in Younes et al. (2006). Use in IHC reported in scientific literature (PMID 27740879). Use in Flow-cell surface reported in scientific literature (PMID: 16426839).
Mouse reactivity reported in scientific literature (PMID: 21474767).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for TRAIL R2/TNFRSF10B Antibody
death domain containing receptor for TRAIL/Apo-2L
Death receptor 5
KILLERapoptosis inducing protein TRICK2A/2B
TNF-related apoptosis-inducing ligand receptor 2
TRAIL receptor 2
TRAIL-R2apoptosis inducing receptor TRAIL-R2
TRAILR2cytotoxic TRAIL receptor-2
tumor necrosis factor receptor superfamily member 10B
tumor necrosis factor receptor superfamily, member 10b
tumor necrosis factor receptor-like protein ZTNFR9
Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Total cell lysates from T47D and MDA-MB-231 cells were subjected to western blot. PVDF membrane were probed with 2.5 mm/ml Human TRAIL R2 (NB100-56618). A specific band was detected for TRAIL R2 at approximately 48~50 kDa. This experiment was conducted under reducing conditions
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