TRAIL R1/TNFRSF10A Antibody (32A1380)

Images

 
Western Blot: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Analysis using Azide Free version of NB100-56747. NBP2-27350 in Daudi cell lysate using DR4 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary ...read more
Western Blot: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Analysis of DR4 in Daudi cell lysate using DR4 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were ...read more
Simple Western: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Simple Western lane view shows a specific band for TRAIL R1 in 0.5 mg/ml of Daudi lysate. This experiment was performed under reducing conditions ...read more
Simple Western: TRAIL R1/TNFRSF10A Antibody (32A1380) [NB100-56747] - Analysis using Azide Free version of NB100-56747. Simple Western lane view shows a specific band for TRAIL R1 in 0.5 mg/ml of Daudi lysate. This ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, CyTOF-ready
Clone
32A1380
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TRAIL R1/TNFRSF10A Antibody (32A1380) Summary

Immunogen
This monoclonal antibody was raised against a peptide corresponding to amino acid 2 to 21 of human DR4.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF10A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Simple Western 20 ug/ml
  • Flow Cytometry 1:20-1:2000
  • Immunocytochemistry/Immunofluorescence
  • CyTOF-ready
Application Notes
A 57 kDa band should be observed. This antibody is routinely tested be western blot analysis. For other applicatons, please refer to appropriate published references for more information. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26044191).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Publications
Read Publications using
NB100-56747 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TRAIL R1/TNFRSF10A Antibody (32A1380)

  • APO2
  • CD261 antigen
  • CD261
  • cytotoxic TRAIL receptor
  • Death receptor 4
  • DR4 TRAIL receptor 1
  • DR4
  • TNF-related apoptosis inducing ligand receptor 1
  • TNF-related apoptosis-inducing ligand receptor 1
  • TNFRSF10A
  • TRAIL R1
  • TRAILR1
  • TRAIL-R1
  • TRAILR-1
  • TRAILR1MGC9365
  • tumor necrosis factor receptor superfamily member 10A
  • tumor necrosis factor receptor superfamily, member 10a

Background

Cells undergo programmed cell death (Apoptosis) in response to various stimuli. Apoptosis is essential for morphogenesis, tissue homeostasis, and host defense Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1). DR4 is expressed in most of human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kB activation in many tissues and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TRAIL R1/TNFRSF10A Antibody (NB100-56747)(20)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Flow, Flow - CS, Flow-IC, ICC/IF, WB.


Filter By Application
Flow
(1)
Flow - CS
(1)
Flow-IC
(1)
ICC/IF
(1)
WB
(4)
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Filter By Species
Human
(6)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB100-56747 Applications Species
Foster KA, Jane EP, Premkumar DR et al. NVP-BKM120 potentiates apoptosis in tumor necrosis factor-related apoptosis-inducing ligand-resistant glioma cell lines via upregulation of Noxa and death receptor 5 Int. J. Oncol. 2015 Jun 05 [PMID: 26044191] (ICC/IF, Human) ICC/IF Human
Qiu F, Hu M, Tang B, Liu X et al. Annexin V-TRAIL fusion protein is a more sensitive and potent apoptotic inducer for cancer therapy. Sci Rep 2013 Dec 20 [PMID: 24356445] (WB, Human) WB Human
Abdulghani Junaid, Allen Joshua E, Dicker David T et al. Sorafenib sensitizes solid tumors to Apo2L/TRAIL and Apo2L/TRAIL receptor agonist antibodies by the Jak2-Stat3-Mcl1 axis. PLoS One. 2013 [PMID: 24086526] (Human) Human
Park S, Cho DH, Andera L et al. Curcumin enhances TRAIL-induced apoptosis of breast cancer cells by regulating apoptosis-related proteins. Mol Cell Biochem 2013 Nov [PMID: 23846485] (WB, Human) WB Human
Allen JE, Ferrini R, Dicker DT et al. Targeting TRAIL death receptor 4 with trivalent DR4 Atrimer complexes. Mol Cancer Ther. 2012 Oct [PMID: 22802267]

Details:
Antibodies cited: 1. DR5 (IMG-120A): Flow (cell surface) 2. DR4 (IMG-141): Flow (cell surface, HCT116 cells), Supplementary Fig. 5. 3. DcR2 (IMG-121-1): WB (SW620 and HCT15 cells), Supplementary Fig. 4.
Jane EP, Premkumar DR, Pollack IF. Bortezomib sensitizes malignant human glioma cells to TRAIL, mediated by inhibition of the NF-{kappa}B signaling pathway. Mol Cancer Ther. 2011 Jan [PMID: 21220502] (WB)

Details:
DR4 (IMG-141A) and DR5/TRAILR2 (IMG-120A). WB: Various human glioma cell lines treated with TRAIL: LN18, U87, LNZ308, and U373, Fig 2B & 2C.
WB
Lin YD, Chen S, Yue P et al. CAAT/enhancer binding protein homologous protein-dependent death receptor 5 induction is a major component of SHetA2-induced apoptosis in lung cancer cells. Cancer Res. 2008 Jul 1 [PMID: 18593935] (WB)

Details:
Antibodies cited [WB ( human non-small cell lung cancer cell lines)]: 1. Anti-caspase-3 (IMG-144A): Figs. 1A,B; 5A. 2. DR4 (NT) TRAIL-R1 (IMG-141A): Figs. 3A.
WB
Le Poole IC, ElMasri WM, Denman CJ et al. Langerhans cells and dendritic cells are cytotoxic towards HPV16 E6 and E7 expressing target cells. Cancer Immunol Immunother. 2008 Jun [PMID: 18004565] (Flow - CS, Human)

Details:
Flow (cell surface), human dendritic cells and langerhans cells, Fig. 4A.
Flow - CS Human
Tsurushima H, Yuan X, Dillehay LE, Leong KW. Radioresponsive tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) gene therapy for malignant brain tumors. Cancer Gene Ther. 2007 Aug [PMID: 17541421] (Flow-IC, Human)

Details:
DR4 (IMG-141): Flow (Cell Surface) and Flow (intracellular), Fig 4a (U251 human glioma cells) & Fig 4c (U87 human glioma cells).
Flow-IC Human
Lee SH, Kim YK, Kim CS et al. E2 of hepatitis C virus inhibits apoptosis. J Immunol. 2005 Dec 15 [PMID: 16339562]
Show All 20 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NBP2-27350APC
DyLight 350 Labeled NBP2-27350UV
DyLight 405 Labeled NBP2-27350V
DyLight 488 Labeled NBP2-27350G
DyLight 550 Labeled NBP2-27350R
DyLight 650 Labeled NBP2-27350C
DyLight 680 Labeled NBP2-27350FR
DyLight 755 Labeled NBP2-27350IR
HRP Labeled NBP2-27350H
PE Labeled NBP2-27350PE
PerCP Labeled NBP2-27350PCP

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Bioinformatics

Gene Symbol TNFRSF10A
Entrez
Uniprot