TRAF-6 Antibody

Images

 
Western Blot: TRAF-6 Antibody [H00007189-D01P] - Analysis of TRAF6 expression in human kidney.
Western Blot: TRAF-6 Antibody [H00007189-D01P] - Analysis of TRAF6 expression in transfected 293T cell line by TRAF6 polyclonal antibody.Lane 1: TRAF6 transfected lysate(59.60 KDa).Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

TRAF-6 Antibody Summary

Immunogen
TRAF6 (AAH31052.1, 1 a.a. - 522 a.a.) full-length human protein. MSLLNCENSCGFSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTAKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRSTDAGV
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRAF6
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
It has been used for WB.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein G purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TRAF-6 Antibody

  • E3 ubiquitin-protein ligase TRAF6
  • EC 6.3.2.-
  • Interleukin-1 signal transducer
  • RING finger protein 85
  • RNF85MGC:3310
  • TNF receptor-associated factor 6
  • TRAF6
  • TRAF-6

Background

TRAFs (Tumor necrosis factor receptor-associated factors) are cytoplasmic adaptor proteins that associate with tumor necrosis factor receptors (TNFR), thus mediating tumor necrosis factor (TNF) induced signaling. The TRAF family consists of six members (TRAF1-6).1-3 TRAF proteins play an important role in regulating cellular death and cellular response to stress by mediating stimulation of AP-1 family through activation of MAP kinases, including JNK/SAPKs, ERKs and p38s.4 They are also involved in the regulation of inflammatory and anti-apoptotic responses through activation of the transcription factors of NF-kB.5 TRAF6 possesses several binding motifs in CD40, TRANCE-R and IRAK suggesting a crucial role in TRANCE-R signaling. It is involved in the activation of the anti-apoptotic serine/threonine kinase Akt/PKB through TRANCE-R.6 Due to its specific receptor-binding TRAF-C domain, TRAF6 is the only member of the TRAF family that also participates in signal transduction of the interlukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily.1,7,8 TRAF6 plays a crucial role in adaptive immunity, innate immunity, bone homeostasis, perinatal and postnatal survival, and cytokine signaling.9 Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Reacts with the human and mouse TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids). Reactivity has been confirmed with human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates.Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TRAF6
Entrez
Uniprot