TRAF-2 Antibody

Product Discontinued

TRAF-2 Antibody Summary

• Immunogen: Peptide with sequence C-KMEAKNSYVRDD corresponding to C-Terminus according to NP_066961.2.
• Epitope: C-KMEAKNSYVRDD
• Clonality: Polyclonal
• Host: Goat
• Gene: TRAF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.5 ug/mL
• Application Notes: WB: Approx. 50 kDa band observed in human ovary and mouse testis lysates and in lysates of cell line HepG2 (calculated MW of 55.9 kDa band according to NP_066961.2).
• Control:

  Human Ovary Whole Tissue Lysate (Adult Whole Normal)
  TRAF-2 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRAF-2 Antibody

• E3 ubiquitin-protein ligase TRAF2
• EC 6.3.2.-
• MGC:45012
• TNF receptor-associated factor 2
• TRAF2
• TRAF-2
• TRAP3
• TRAP3TRAP
• tumor necrosis factor type 2 receptor associated protein 3
• Tumor necrosis factor type 2 receptor-associated protein 3

Background

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-2 Antibody (NB100-58959)(1)

Publication using NB100-58959

• Xu Y, Huang S, Liu ZG, Han J. Poly(ADP-ribose) polymerase-1 signaling to mitochondria in necrotic cell death requires RIP1/TRAF2-mediated JNK1 activation. J Biol Chem 2006-01-30 [PMID: 16446354]

Bioinformatics

• Gene Symbol: TRAF2
• Entrez:
  • 7186(Human)
  • 22030(Mouse)
• Uniprot:
  • NP_066961.2(Human)