Trace Amine Receptor 1 Antibody

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Western Blot: Trace Amine Receptor 1 Antibody [NB100-68145] - NB100-68142 (0.03ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-68145
    • Availability
      Product Discontinued

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Trace Amine Receptor 1 Antibody Summary

Immunogen
Synthetic peptide, C-EGKSQAPQSKETK, representing the internal region of the sequence according to NP_444435.1
Epitope
EGKSQAPQSKETK
Clonality
Polyclonal
Host
Goat
Gene
TAAR1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.03ug/ml
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Preliminary experiments gave bands at approx 38kDa, but also at approx 90kDa and 75kDa in lysates of Mouse Brain and of cell line K562 after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 39.1kDa according to NP_444435.1. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reactivity Notes

Mouse and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Trace Amine Receptor 1 Antibody

  • MGC126874
  • MGC138399
  • TA1RP11-295F4.9
  • TaR-1
  • TAR1trace amine-associated receptor 1
  • trace amine associated receptor 1
  • Trace amine receptor 1taR-1
  • TRAR1

Background

TA1 (Trace amine receptor 1) is a member of the G protein coupled receptor family (subfamily trace amine). It is activated by endogenous trace amines as well as metabolites of the biogenic amine neurotransmitters. Trace amines are biogenic amines present in very low levels in mammalian tissues. Although some trace amines have clearly defined roles as neurotransmitters in invertebrates, the extent to which they function as true neurotransmitters in vertebrates has remained speculative. Trace amines are likely to be involved in a variety of physiological functions that have yet to be fully understood. This receptor is mediated by the G(s)-class of G-proteins which activate adenylate cyclase. TA1 has been reported in human brain, dorsal root ganglion, olfactory bulb, kidney, liver, lung, pancreas, prostate, skeletal muscle, small intestine, spleen, spinal cord, and stomach. An EST for TA1 has been identified from a human stomach cancer library.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TAAR1
Entrez
Uniprot