TRA-1-60® Antibody (TRA-1-60)

Product Discontinued

TRA-1-60® Antibody (TRA-1-60) Summary

• Immunogen: Human embryonal carcinoma cell line 2102Ep.
• Localization: Cell Membrane
• Marker: Embryonic Stem Cell Marker
• Specificity: Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. TRA-1-60 reacts with a sialidase-sensitive epitope.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: This TRA-1-60 (R) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western blot.
• Control:

  PAR4 Antibody

Reactivity Notes

Human. No immunoreactivity is seen with murine EC, EG or ES cells.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: IgM purified

Alternate Names for TRA-1-60(R) Antibody (TRA-1-60)

• TRA160(R)
• TRA-1-60(R)

Background

TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.