TRA-1-60® Antibody (TRA-1-60)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-730-PBSonly
    • Availability
      Product Discontinued

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TRA-1-60® Antibody (TRA-1-60) Summary

Immunogen
Human embryonal carcinoma cell line 2102Ep.
Localization
Cell Membrane
Marker
Embryonic Stem Cell Marker
Specificity
Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. TRA-1-60 reacts with a sialidase-sensitive epitope.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
IgM purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:50 - 1:200
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunoprecipitation 1:10 - 1:500
Application Notes
This TRA-1-60 (R) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western blot.
Control
PAR4 Antibody

Reactivity Notes

Human. No immunoreactivity is seen with murine EC, EG or ES cells.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
IgM purified

Alternate Names for TRA-1-60(R) Antibody (TRA-1-60)

  • TRA160(R)
  • TRA-1-60(R)

Background

TRA-1-60 is a cell-surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis. The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors. Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan. Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional TRA-1-60(R) Products

Array NB100-730-PBSonly

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