Recombinant Human TPK1 Protein (NBP1-99102)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human TPK1 Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 243 of Human TPK1

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACAD GGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKD LKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLI PVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS
Preparation
Method
E.coli
Protein/Peptide Type
Recombinant Protein
Gene
TPK1
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 29.8 kDa (267aa) confirmed by MALDI-TOF
Theoretical MW
29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human TPK1 Protein

  • EC 2.7.6.2
  • HTPK1
  • Placental protein 20
  • PP20thiamine kinase
  • thiamin pyrophosphokinase 1
  • thiamine diphosphokinase
  • Thiamine pyrophosphokinase 1

Background

TPK1, also known as thiamin pyrophosphokinase 1, catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. This protein can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Recombinant human TPK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for TPK1 Protein (NBP1-99102). (Showing 1 - 1 of 1 FAQ).

  1. We are trying to get activity out of this enzyme (thiamine pyrophosphokinase) and are having difficulty. Do you guys have any recommendations that may help us out?
    • I am sorry for any confusion regarding our protein product NBP1-99102. This antibody is sold with a reducing agent present in the buffer as the protein is advertised for use in SDS PAGE gels as a positive control. Our lab has not yet tested this protein for use in functional assays that require activity. 

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Bioinformatics

Gene Symbol TPK1

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