TOR/mTOR [p Ser2448] Antibody

Images

 
Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP1-51413] - Analysis of mTOR (p-S2448) pAb at 1:500 dilutionLane1:L02 whole cell lysateLane2:L02 treated with EGF(0.1ng/ml,5min) whole cell lysateLane3:L02 treated with ...read more
Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NBP1-51413] - Human brain tissue.
Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP1-51413] - Extracts from A549 and Hela cells.
Western Blot: TOR/mTOR [p Ser2448] Antibody [NBP1-51413] - Analysis of p-mTOR (S2448) pAb at 1:500 dilutionLane1:HEK293T cell lysate treated with insulin(100nM,30mins)Lane2:Raw264.7 cell lysate treated with ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-51413
    • Availability
      Product Discontinued

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TOR/mTOR [p Ser2448] Antibody Summary

Immunogen
Synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Serine 2442.
Modification
p Ser2448
Specificity
p-mTOR (S2448) pAb detects endogenous levels of mTOR protein when phosphorylated at Ser2448.
Clonality
Polyclonal
Host
Rabbit
Gene
MTOR
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of p-mTOR (S2448) pAb in extracts from A549 and Hela cells.
Immunohistochemistry (IHC) analyzes of p-mTOR (S2448) pAb in paraffin-embedded human brain tissue.
Theoretical MW
289 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TOR/mTOR [p Ser2448] Antibody

  • EC 2.7.11.1
  • FK506 binding protein 12-rapamycin associated protein 1
  • FK506 binding protein 12-rapamycin associated protein 2
  • FK506-binding protein 12-rapamycin complex-associated protein 1
  • FKBP12-rapamycin complex-associated protein
  • FLJ44809
  • FRAP
  • FRAP1
  • FRAP1FKBP12-rapamycin complex-associated protein 1
  • FRAP2
  • Mammalian target of rapamycin
  • mechanistic target of rapamycin (serine/threonine kinase)
  • Mechanistic target of rapamycin
  • MTOR
  • RAFT1
  • rapamycin and FKBP12 target 1
  • rapamycin associated protein FRAP2
  • Rapamycin target protein 1
  • RAPT1FKBP-rapamycin associated protein
  • serine/threonine-protein kinase mTOR
  • TOR

Background

mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TOR/mTOR Antibody (NBP1-51413)

Discover related pathways, diseases and genes to TOR/mTOR Antibody (NBP1-51413). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on TOR/mTOR.

Thomson Reuters Predicts 2016 Nobel Prize Winners
Here at Bio-Techne we always look forward to the annual announcements of winners of the highly coveted Nobel Prize – the greatest award in science. How can you go about predicting which scientists might be in line for a life-changing phone-call fro...  Read full blog post.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

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Bioinformatics

Gene Symbol MTOR
Entrez