TOR/mTOR [p Ser2448] Antibody

Product Discontinued

TOR/mTOR [p Ser2448] Antibody Summary

• Immunogen: Synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Serine 2442.
• Modification: p Ser2448
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTOR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
• Application Notes: Immunohistochemistry (IHC) analyzes of p-mTOR (S2448) pAb in paraffin-embedded human brain tissue.
• Theoretical MW: 289 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross reacts with Human, Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (approx. pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TOR/mTOR [p Ser2448] Antibody

• EC 2.7.11.1
• FK506 binding protein 12-rapamycin associated protein 1
• FK506 binding protein 12-rapamycin associated protein 2
• FK506-binding protein 12-rapamycin complex-associated protein 1
• FKBP12-rapamycin complex-associated protein
• FLJ44809
• FRAP
• FRAP1
• FRAP1FKBP12-rapamycin complex-associated protein 1
• FRAP2
• Mammalian target of rapamycin
• mechanistic target of rapamycin (serine/threonine kinase)
• Mechanistic target of rapamycin
• MTOR
• RAFT1
• rapamycin and FKBP12 target 1
• rapamycin associated protein FRAP2
• Rapamycin target protein 1
• RAPT1FKBP-rapamycin associated protein
• serine/threonine-protein kinase mTOR
• TOR

Background

mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOR/mTOR Antibody (NBP1-19934)(1)

Publication using NBP1-19934

• Zhang S, Cao F, Li W, Abumaria N TRPM7 kinase activity induces amyloid-? degradation to reverse synaptic and cognitive deficits in mouse models of Alzheimer's disease Science signaling 2023-07-11 [PMID: 37433006] (WB, Mouse)

Bioinformatics

• Gene Symbol: MTOR
• Entrez:
  • 2475(Human)
  • 56717(Mouse)
  • 56718(Rat)