TOP2A Antibody

Images

 
Western Blot: TOP2A Antibody [NBP1-01040] - Analysis of TOP2A (Q14) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate
Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-01040] - Analyzes of paraffin-embedded human breast carcinoma tissue at 1:100.
Western Blot: TOP2A Antibody [NBP1-01040] - Topo II Alpha Antibody Western blot (WB) analysis of Topo IIalpha (Q14) antibody in extracts from HT-29.
Immunohistochemistry: TOP2A Antibody [NBP1-01040] - Topo II Alpha Antibody Immunohistochemistry (IHC) analyzes of Topo IIalpha (Q14) antibody in paraffin-embedded human liver carcinoma tissue

Product Details

Summary
Product Discontinued
View other related TOP2A Primary Antibodies

Order Details


    • Catalog Number
      NBP1-01040
    • Availability
      Product Discontinued

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TOP2A Antibody Summary

Immunogen
Synthetic peptide, corresponding to the N-terminus of Human Topo IIalpha.
Clonality
Polyclonal
Host
Rabbit
Gene
TOP2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Topo IIalpha (Q14) pAb in extracts from HT-29.
Immunohistochemistry (IHC) analyzes of Topo IIalpha (Q14) pAb in paraffin-embedded human liver carcinoma tissue.
Theoretical MW
174 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TOP2A Antibody

  • DNA topoisomerase (ATP-hydrolyzing)
  • DNA topoisomerase 2-alpha
  • DNA topoisomerase II, 170 kD
  • DNA topoisomerase II, alpha isozyme
  • EC 5.99.1.3
  • TOP2
  • TOP2A
  • TOP2DNA gyrase
  • topoisomerase (DNA) II alpha (170kD)
  • topoisomerase (DNA) II alpha 170kDa
  • TP2A

Background

DNA topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing both positive and negative supercoils, whereas prokaryotic topoisomerases relax only negative supercoils. DNA topoisomerases play a role in DNA replication, recombination, and transcription and have been identified as targets of numerous anticancer drugs. Topo I, a ubiquitously expressed, soluble enzyme, acts by introducing a transient break in one strand of DNA, while Topo II acts by making a transient double-strand break. Topo II is encoded by two different genes to generate two distinct isoforms that are designated Topo IIalpha and Topo IIbeta. The 180 kDa isoform, Topo IIbeta, and the 170 kDa isoform, Topo IIalpha, are largely homologous at their N-terminal three quarters, however, the C-terminal segments are considerably divergent, suggesting that these regions may mediate different cellular functions and account for the observed differential tissue expression patterns of the two isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TOP2A
Entrez