TOM70 Antibody

Images

 
Western Blot: TOM70 Antibody [NBP1-76969] - Analysis in mouse brain tissue lysate with antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: TOM70 Antibody [NBP1-76969] - Immunofluorescence of TOM70 in Mouse Brain cells with TOM70 antibody at 5 ug/mL.
Immunohistochemistry-Paraffin: TOM70 Antibody [NBP1-76969] - Mouse brain tissue with TOM70 antibody at 2.5 ug/ml.
Western Blot: TOM70 Antibody [NBP1-76969] - Analysis of 293 cell lysate with TOM70 antibody at 2 ug/ml.

Product Details

Summary
Product Discontinued
View other related TOM70 Primary Antibodies

Order Details


    • Catalog Number
      NBP1-76969
    • Availability
      Product Discontinued

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TOM70 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOM70. The immunogen is located within amino acids 50 - 100 of TOM70.
Specificity
At least two isoforms of TOM70 are known to exist; this antibody will only detect the larger isoform.
Clonality
Polyclonal
Host
Rabbit
Gene
TOMM70A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Reactivity Notes

Human, Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TOM70 Antibody

  • FLJ90470
  • KIAA0719
  • mitochondrial import receptor subunit TOM70
  • Mitochondrial precursor proteins import receptor
  • TOM70
  • Translocase of outer membrane 70 kDa subunit
  • translocase of outer mitochondrial membrane 70 (yeast) homolog A
  • translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
  • translocase of outer mitochondrial membrane 70 homolog A (yeast)

Background

The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear. At least two isoforms of TOM70 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TOM70 Antibody (NBP1-76969)

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Bioinformatics

Gene Symbol TOMM70A
Uniprot