TNIP1 Antibody (5C4)

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Western Blot: TNIP1 Antibody (5C4) [NBP1-43137] - Immunoblotting of lysates prepared from Jurkat cells with 5 ug/mL Anti-Human Naf1 Purified. Bands were visualized using Anti-Mouse IgG HRP.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43137
    • Availability
      Product Discontinued

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TNIP1 Antibody (5C4) Summary

Immunogen
The immunogen for this antibody was Naf1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNIP1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The 5C4 antibody has been tested by western blot on lysates prepared from Jurkat cells. This antibody can be used at 1-5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performace in the assay of interest.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for TNIP1 Antibody (5C4)

  • ABIN-1
  • HIV-1 Nef-interacting protein
  • hVAN
  • KIAA0113nip40-1
  • Naf1
  • NAF1TNFAIP3-interacting protein 1
  • Nef-associated factor 1 SNP
  • Nef-associated factor 1
  • Nip40-1
  • TNFAIP3 interacting protein 1
  • VANvirion-associated nuclear-shuttling protein
  • Virion-associated nuclear shuttling protein

Background

This 5C4 monoclonal antibody reacts with human Nef-associated factor-1 (Naf-1). This protein is also designated as A20-binding inhibitor of NFkB activation (ABIN-1) and TNFAIP3-interacting protein 1 (TNIP1). Naf-1 is predicted to consist of four leucine zippers and four coiled-coil domains. This protein exists as two isoforms, Naf1alpha and Naf1beta, which are 72-kDa and 68-kDa, respectively, and is expressed in a variety of tissues, with greatest expression in peripheral blood lymphocytes, spleen, and skeletal muscle. Naf1 has also been detected in the Jurkat, Molt-4, and HL60 cell lines. Naf-1 has been shown to play several biological roles. Naf-1 was originally identified associated with the Nef protein of HIV, and was found to enhance CD4 cell surface expression. Naf-1 has also been demonstrated to inhibit TNF-alpha- and IL-1-mediated NFkB-dependent gene expression by competing with IKK-gamma. Finally, this adaptor protein has been found to associate constitutively with P-selectin glycoprotein ligand 1 (PSGL-1) in human leukocytes. Upon P-selectin binding to PSGL-1, Naf1 is phosphorylated at Tyr552 by Src family kinases, leading to PI3K recruitment and integrin activation. This antibody recognizes both the unphosphorylated and phosphorylated forms of Naf1alpha and Naf1beta.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TNIP1