TNIP1 Antibody (5C4)


Western Blot: TNIP1 Antibody (5C4) [NBP1-43137] - Immunoblotting of lysates prepared from Jurkat cells with 5 ug/mL Anti-Human Naf1 Purified. Bands were visualized using Anti-Mouse IgG HRP.

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
0.5 mg/ml

TNIP1 Antibody (5C4) Summary

The immunogen for this antibody was Naf1.
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Western Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The 5C4 antibody has been tested by western blot on lysates prepared from Jurkat cells. This antibody can be used at 1-5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performace in the assay of interest.

Alternate Names for TNIP1 Antibody (5C4)

  • ABIN-1
  • HIV-1 Nef-interacting protein
  • hVAN
  • KIAA0113nip40-1
  • Naf1
  • NAF1TNFAIP3-interacting protein 1
  • Nef-associated factor 1 SNP
  • Nef-associated factor 1
  • Nip40-1
  • TNFAIP3 interacting protein 1
  • VANvirion-associated nuclear-shuttling protein
  • Virion-associated nuclear shuttling protein


This 5C4 monoclonal antibody reacts with human Nef-associated factor-1 (Naf-1). This protein is also designated as A20-binding inhibitor of NFkB activation (ABIN-1) and TNFAIP3-interacting protein 1 (TNIP1). Naf-1 is predicted to consist of four leucine zippers and four coiled-coil domains. This protein exists as two isoforms, Naf1alpha and Naf1beta, which are 72-kDa and 68-kDa, respectively, and is expressed in a variety of tissues, with greatest expression in peripheral blood lymphocytes, spleen, and skeletal muscle. Naf1 has also been detected in the Jurkat, Molt-4, and HL60 cell lines. Naf-1 has been shown to play several biological roles. Naf-1 was originally identified associated with the Nef protein of HIV, and was found to enhance CD4 cell surface expression. Naf-1 has also been demonstrated to inhibit TNF-alpha- and IL-1-mediated NFkB-dependent gene expression by competing with IKK-gamma. Finally, this adaptor protein has been found to associate constitutively with P-selectin glycoprotein ligand 1 (PSGL-1) in human leukocytes. Upon P-selectin binding to PSGL-1, Naf1 is phosphorylated at Tyr552 by Src family kinases, leading to PI3K recruitment and integrin activation. This antibody recognizes both the unphosphorylated and phosphorylated forms of Naf1alpha and Naf1beta.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC-P, IP
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Applications: Func-Inh
Species: Hu, Mu, Rt, Bv, ChHa, Dr, Fu, Pl, Pr, Rb, Sh, Xp, Ye, Ze
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB
Species: Hu
Applications: WB, IHC
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu
Applications: WB, Simple Western, ICC
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: In vitro, In vivo, Func-Inh
Species: Hu, Mu, Rt
Applications: WB, IHC
Species: Hu
Applications: WB, IHC, IHC-P, IP

Publications for TNIP1 Antibody (NBP1-43137) (0)

There are no publications for TNIP1 Antibody (NBP1-43137).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TNIP1 Antibody (NBP1-43137) (0)

There are no reviews for TNIP1 Antibody (NBP1-43137). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TNIP1 Antibody (NBP1-43137) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional TNIP1 Antibody (5C4) Products

Related Products by Gene

Bioinformatics Tool for TNIP1 Antibody (NBP1-43137)

Discover related pathways, diseases and genes to TNIP1 Antibody (NBP1-43137). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TNIP1 Antibody (NBP1-43137)

Discover more about diseases related to TNIP1 Antibody (NBP1-43137).

Pathways for TNIP1 Antibody (NBP1-43137)

View related products by pathway.

PTMs for TNIP1 Antibody (NBP1-43137)

Learn more about PTMs related to TNIP1 Antibody (NBP1-43137).

Blogs on TNIP1

There are no specific blogs for TNIP1, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol TNIP1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-43137 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought