TNFAIP8 Antibody

Western Blot: TNFAIP8 Antibody [NBP3-00317] - Anti-TNFAIP8 rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 33 kDa

Immunohistochemistry-Paraffin: TNFAIP8 Antibody [NBP3-00317] - Immunochemical staining of human TNFAIP8 in human spleen with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).

Immunohistochemistry-Paraffin: TNFAIP8 Antibody [NBP3-00317] - Immunochemical staining of human TNFAIP8 in human lung cancer with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).

Immunoprecipitation: TNFAIP8 Antibody [NBP3-00317] - TNFAIP8 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate0.5 ul anti-TNFAIP8 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TNFAIP8 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 17 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

TNFAIP8 Antibody Summary

• Immunogen: Produced in rabbits immunized with purified, recombinant Human TNFAIP8 (O95379; Met1-Ile198).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNFAIP8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Affinity purified

Notes

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for TNFAIP8 Antibody

• GG2-1
• Head and neck tumor and metastasis-related protein
• MDC-3.13TNF-induced protein GG2-1
• NDED
• NF-kappa-B-inducible DED-containing protein
• SCC-S2SCCS2
• TNF alpha-induced protein 8
• tumor necrosis factor alpha-induced protein 8
• tumor necrosis factor, alpha-induced protein 8

Background

Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFAIP8