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TNF RII/TNFRSF1B Antibody (101)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Clone
101
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-90433AF350
Alexa Fluor 405
NBP2-90433AF405
Alexa Fluor 488
NBP2-90433AF488
Alexa Fluor 532
NBP2-90433AF532
Alexa Fluor 594
NBP2-90433AF594
Alexa Fluor 647
NBP2-90433AF647
Alexa Fluor 700
NBP2-90433AF700
Alexa Fluor 750
NBP2-90433AF750
Biotin
NBP2-90433B
DyLight 350
NBP2-90433UV
DyLight 405
NBP2-90433V
DyLight 488
NBP2-90433G
DyLight 550
NBP2-90433R
DyLight 594
NBP2-90433DL594
DyLight 650
NBP2-90433C
DyLight 680
NBP2-90433FR
DyLight 755
NBP2-90433IR
FITC
NBP2-90433F
HRP
NBP2-90433H
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TNF RII/TNFRSF1B Antibody (101) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse TNF RII/TNFRSF1B (NP_033491.2; Met1-Gly258).
Specificity
Has cross-reactivity in ELISA with:

Human TNFRII
No cross-reactivity in ELISA with:

Mouse CD27
Human DR6
Mouse FAS
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TNFRSF1B
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:5000-1:10000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for TNF RII/TNFRSF1B Antibody (101)

  • CD120b antigen
  • CD120b
  • Etanercept
  • p75 TNF receptor
  • p75TBPII
  • p75TNFR
  • soluble TNFR1B variant 1
  • TNF RII
  • TNFBRp80 TNF-alpha receptor
  • TNF-R2
  • TNFR2TNFR1B
  • TNF-R75
  • TNFR80
  • TNFRII
  • TNF-RII
  • TNF-R-II
  • TNFR-II
  • TNFRSF1B
  • tumor necrosis factor beta receptor
  • tumor necrosis factor binding protein 2
  • Tumor necrosis factor receptor 2
  • tumor necrosis factor receptor superfamily member 1B
  • tumor necrosis factor receptor superfamily, member 1B
  • Tumor necrosis factor receptor type II

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
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NGF R/TNFRSF16 was detected in immersion fixed canine mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
NGF R/TNFRSF16 was detected in immersion fixed human mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
NGF R/TNFRSF16 was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 25 µg/mL Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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BDNF [HRP]
DBD00
(4 Reviews)
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CD25/IL-2R alpha
[HRP]
DR2A00
(33 Publications)
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Applications: ELISA
c-Fos Antibody
(2H2)
NBP2-50037
Knockout Validated: c-Fos Antibody (2H2) [NBP2-50037] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and c-Fox knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Mouse Anti-Human c-Fox Monoclonal Antibody (Catalog # NBP2-50037) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for c-Fox at approximately 52 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
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Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
Protein Disulfide
Isomerase/P4HB Antibo ...
NB300-517
Western Blot: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Cell surface Protein Disulfide Isomerase/P4HB associated with GC-AC. Proteins were pulled down with anti GC-A antibody from HUVEC or HMC membrane preparations and detected with anti-Protein Disulfide Isomerase/P4HB antibody (RL90)compared to control IP (no anti GC-A) and non-immunoprecipitated proteins from HMC and HUVEC. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0112986), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Protein Disulfide Isomerase/P4HB monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 at a concentration of 1ug/ml for 30 minutes.
Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody (RL90) [NB300-517] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Protein Disulfide Isomerase/P4HB monoclonal antibody at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes.
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Fascin Antibody
(833223) [Unconjugate
MAB7745
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Fascin at approximately 61 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fascin knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fascin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockoutHeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. New adjunct appears with knockout cell line.
Fascin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Fascin Monoclonal Antibody (Catalog # MAB7745) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to synusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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PI 3-Kinase p55
gamma Antibody (SR44- ...
NBP2-67329
Immunocytochemistry/Immunofluorescence: PI 3-Kinase p55 gamma Antibody (SR44-06) [NBP2-67329] - Staining PI 3 Kinase p55 ? in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PI 3-Kinase p55 gamma Antibody (SR44-06) [NBP2-67329] - Analysis of paraffin-embedded mouse brain tissue using anti-PI 3 Kinase p55 ? antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: PI 3-Kinase p55 gamma Antibody (SR44-06) [NBP2-67329] - Analysis of paraffin-embedded mouse testis tissue using anti-PI 3 Kinase p55 ? antibody. Counter stained with hematoxylin.
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Applications: ICC/IF, IHC, IHC-P, WB
ERG Antibody - BSA
Free
NBP2-60655
Immunocytochemistry/Immunofluorescence: ERG Antibody [NBP2-60655] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ERG at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: ERG Antibody [NBP2-60655] - Total protein from Human THP-1, Jurkat and K562 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ERG in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: ERG Antibody [NBP2-60655] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human kidney (NBP1-78269) using 1:100 dilution of ERG antibody. The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a nuclear staining with weak cytoplasmic positivity in the cells of the glomeruli and the tubular epithelial cells.
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POLG2 Antibody
NBP2-94064
Immunocytochemistry/Immunofluorescence: POLG2 Antibody [NBP2-94064] - Analysis of MCF7 cells using POLG2 . Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: POLG2 Antibody [NBP2-94064] - Human stomach using POLG2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: POLG2 Antibody [NBP2-94064] - Mouse kidney using POLG2 antibody at dilution of 1:100 (40x lens).
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MPP1 Antibody
(1E11-1G11)
H00004354-M01
Western Blot: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of monoclonal antibody to MPP1 on HeLa cell . Antibody concentration 10 ug/ml.
Immunoprecipitation: MPP1 Antibody (1E11-1G11) [H00004354-M01] - Analysis of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MPP1 monoclonal antibody.
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PSMD12 Antibody
NBP1-87801
Immunocytochemistry/Immunofluorescence: PSMD12 Antibody [NBP1-87801] - Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.
Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801] - Staining of human salivary gland shows low expression as expected.
Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801] - Staining in human testis and salivary gland tissues using anti-PSMD12 antibody. Corresponding PSMD12 RNA-seq data are presented for the same tissues.
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TrkA Antibody
[Unconjugated]
AF1056
TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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CTLA-4
[Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
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NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
(1 Review)
(23 Publications)
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Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.
Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).
Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
IL-2 [Unconjugated]
202-IL
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(26 Reviews)
(342 Publications)
Species: Hu
Applications: BA
beta-NGF
[Unconjugated]
256-GF/CF
1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human beta -NGF (Catalog # 256‑GF/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.2-2 ng/mL.
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Species: Hu
Applications: BA
TNF RII/TNFRSF1B
Antibody (101)
NBP2-90433
TNF RII/TNFRSF1B Antibody (101)
Species: Mu
Applications: ELISA

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Bioinformatics

Gene Symbol TNFRSF1B