TNF-alpha Partial Recombinant Protein


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Product Details

Reactivity MuSpecies Glossary
Applications ELISA, IA
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

TNF-alpha Partial Recombinant Protein Summary

A partial protein corresponding to TNF.
E. coli expressed amino acids Leu 80-Leu 235 of mouse TNF-a (accession # NM_013693).
Details of Functionality
The ED50 of this protein, as measured by L929 cytotoxicity assay, is less than or equal to 25 pg/mL. This corresponds to a specific activity of greater than or equal to 4 x 10^7 Units/mg.
Endotoxin Note
Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with no carriers. 0.22 um filtered.
No Preservative
Please see the vial label for concentration. If unlisted please contact technical services.


Application Notes
The recombinant mouse TNF-alpha; has been tested by sandwich ELISA using the mouse TNF-alpha; ELISA antibody pair (TN3-19.12/polyclonal antibody).

Reactivity Notes

This protein was made to the mouse sequence.


Purity: Greater than 98%, as determined by SDS-PAGE

Alternate Names for TNF-alpha Partial Recombinant Protein

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


Mouse TNF-a is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-alpha is expressed as a membrane-bound molecule. TNF-alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-alpha have been observed.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Product General Protocols

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FAQs for TNF-alpha Protein (NBP1-42899). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Product PDFs


Gene Symbol TNF

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