Mouse TNF-alpha ELISA Kit (Colorimetric)

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ELISA: Mouse TNF-alpha ELISA Kit (Colorimetric) [NBP2-31085] - Standard Curve

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatants, Plasma (EDTA, Citrate), Serum
Standard Curve Range
31.3 - 2000 pg/mL
Sensitivity
3.7 pg/ml

Order Details

Mouse TNF-alpha ELISA Kit (Colorimetric) Summary

Standard Curve Range
31.3 - 2000 pg/mL
Sensitivity
3.7 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
5.7%
Intra-Assay
6.5%
Spike Recovery
Please See Protocol for Specific Spike Recovery Details
Sample Volume
50 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
TNF

Applications/Dilutions

Application Notes
The mouse TNF-alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TNF-alpha.
Publications
Read Publication using
NBP2-31085 in the following applications:

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  2. Substrate Solution (tetramethyl-benzidine)
  3. Streptavidin-HRP
  4. Stop Solution (1M Phosphoric acid)
  5. Sample Diluent
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. Aluminium pouch(es) with a Microwell Plate coated with polyclonal antibody to mouse TNF-alpha
  8. Adhesive Films
  9. Mouse TNF-alpha Standard lyophilized, 2 ng/mL upon reconstitution
  10. Calibrator Diluent
  11. Biotin-Conjugate anti-mouse TNF-alpha polyclonal antibody

Alternate Names for Mouse TNF-alpha ELISA Kit (Colorimetric)

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

The mouse TNF-alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TNF-alpha.

Platinum Kits undergo stringent in-house development, manufacture and quality-validation processes ensuring highest possible product performance and optimal lot-to-lot consistency.
TNF-alpha release is mainly triggered by viral infections, endotoxins, lipopolysaccharides or other bacterial components, by tissue injury, DNA-damage and by IL-1, PDGF and TNF-alpha itself. It is primarily expressed in macrophages, but also in monocytes, neutrophils, NK-cells, mast-cells, endothelial cells and activated lymphocytes. TNF-alpha expression in endothelial cells and fibroblasts can be induced by IL-17. The expression of other cytokines, chemokines, reactive oxygen intermediates, nitric oxide and prostaglandins is stimulated by TNF-alpha.
Multiple biological functions of TNF-alpha comprise cellular proliferation and differentiation, tumorigenesis, apoptotic or necrotic cell death (including certain tumor cell lines), immunoregulatory activities, lipid metabolism, coagulation and endothelial function. It promotes local or systemic inflammation (TNF-alpha is a potent pyrogen) and stimulates the acute phase response. Very high expressions of TNF-alpha after infection can lead to septic shock (TNF-alpha is highly cytotoxic.), whereas sustained low levels induce cachexia and inflammation. Dysregulation of TNF-alpha is involved in many diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for TNF-alpha ELISA Kit (NBP2-31085)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ELISA.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF-alpha ELISA Kit (NBP2-31085). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Blogs on TNF-alpha.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Bioinformatics

Gene Symbol TNF
Entrez