| Reactivity | MuSpecies Glossary |
| Applications | ELISA |
| Suitable Sample Type | Cell Culture Supernatants, Plasma (EDTA, Citrate), Serum |
| Standard Curve Range | 31.3 - 2000 pg/mL |
| Sensitivity | 3.7 pg/ml |
| Standard Curve Range | 31.3 - 2000 pg/mL |
| Sensitivity | 3.7 pg/ml |
| Assay Type | Sandwich ELISA |
| Inter-Assay | 5.7% |
| Intra-Assay | 6.5% |
| Spike Recovery | Please See Protocol for Specific Spike Recovery Details |
| Sample Volume | 50 ul |
| Kit Type | ELISA Kit (Colorimetric) |
| Gene | TNF |
| Application Notes | The mouse TNF-alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TNF-alpha. |
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| Publications |
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| Storage | Storage of components varies. See protocol for specific instructions. |
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![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
| Publication using NBP2-31085 | Applications | Species |
|---|---|---|
| Wang J, Zhao L, Li Y et al. HuR induces inflammatory responses in HUVECs and murine sepsis via binding to HMGB1 Mol Med Rep. 2018 Jan 01 [PMID: 29115544] (ELISA, Mouse) | ELISA | Mouse |
Diseases for TNF-alpha ELISA Kit (NBP2-31085)Discover more about diseases related to TNF-alpha ELISA Kit (NBP2-31085).
| Pathways for TNF-alpha ELISA Kit (NBP2-31085)View related products by pathway.
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You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t... Read full blog post. |
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Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac... Read full blog post. |
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cIAP2 - balancing cell death and cell survival The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and... Read full blog post. |
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CD86 - I work in tandem with CD80 CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated... Read full blog post. |
| Gene Symbol | TNF |
![ELISA: Mouse TNF-alpha ELISA Kit (Colorimetric) [NBP2-31085] - Standard Curve](http://images.novusbio.com/fullsize2/Mouse-TNF-alpha-ELISA-Kit-(Colorimetric)-ELISA-NBP2-31085-img0002.jpg "ELISA: Mouse TNF-alpha ELISA Kit (Colorimetric) [NBP2-31085] - Standard Curve")
![Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Western-Blot-NB100-56583-img0004.jpg)
![Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Immunohistochemistry-Paraffin-NB100-56583-img0009.jpg)
![Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize2/NFkB-p105-p50-Antibody-(2J10D7)-Flow-Cytometry-NB100-56583-img0008.jpg)
![Western Blot: iNOS Antibody [NB300-605] - Analysis of iNOS was performed by loading 20ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1ug/mL for 16 hours (right lane) and 10ul of PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with an iNOS Rabbit polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBST, and probed with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at a dilution of 1:1000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Western-Blot-NB300-605-img0015.jpg)
![Western Blot: iNOS Antibody [NB300-605] - iNOS in stimulated astrocytes. Image from verified customer review.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Western-Blot-NB300-605-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: iNOS Antibody [NB300-605] - Analysis of iNOS in A549 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a iNOS polyclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. iNOS staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize2/iNOS-Antibody-Immunocytochemistry-Immunofluorescence-NB300-605-img0012.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunocytochemistry-Immunofluorescence-NB100-524-img0008.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Western-Blot-NBP1-84550-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.](http://images.novusbio.com/fullsize2/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0002.jpg)
