The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human TNF alpha positive serum. There was no interference detected, namely not with TNF-R (60 kDa and 80 kDa).
Standard Curve Range
7.8 - 500 pg/mL
The recovery ranged from 72% to 112% with an overall mean recovery of 93%. Please see protocol for more specific Spike Recovery details.
ELISA Kit (Colorimetric)
The human TNF-alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TNF-alpha
Read Publication using NBP1-91170 in the following applications:
Human TNF-alpha Standard lyophilized, 1000 pg/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Alternate Names for Human TNF-alpha ELISA Kit (Colorimetric)
TNFSF2TNF superfamily, member 2
tumor necrosis factor (TNF superfamily, member 2)
tumor necrosis factor alpha
Tumor necrosis factor ligand superfamily member 2
tumor necrosis factor
tumor necrosis factor-alpha
Tumor Necrosis Factor is a polypeptide cytokine produced by monocytes and macrophages. It functions as a multipotent modulator of immune response and further acts as a potent pyrogen. TNF-alpha circulates throughout the body responding to stimuli (infectious agents or tissue injury), activating neutrophils, altering the properties of vascular endothelial cells, regulating metabolic activities of other tissues, as well as exhibiting tumoricidal activity by inducing localized blood clotting. TNF-alpha also inhibits lipoprotein lipase activity resulting in cachexia, a physical wasting condition. Activation of B-cells by the Epstein Barr virus can be inhibited by TNF-alpha. Due to its varied actions throughout the immune system, TNF-alpha may play a role in the pathogenesis of many disease states. TNF-alpha production is mediated by the action of lymphokines and endotoxins on the macrophage. Purified monocytes produce TNF-alpha within four hours of stimulation by recombinant IL-2 and there is some in vitro evidence to suggest that TNF-alpha is expressed at high levels and with prolonged kinetics in T cells stimulated by both CD2 and CD28. Secretion of TNF-alpha is enhanced by gamma interferon. TNF then induces or enhances the specific production of Class I MHC antigen, GM-CSF, and IL-1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
FAQs for TNF-alpha ELISA Kit (NBP1-91170). (Showing 1 - 4 of 4 FAQ).
I need to know about TNF-alpha antibody kits for mice.
We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
CD86 - I work in tandem with CD80 CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated... Read full blog post.