Human TNF-alpha ELISA Kit (Colorimetric)


Sandwich ELISA: Human TNF-alpha ELISA Kit (Colorimetric) [NBP1-83741] - Representative standard curve for human TNF alpha total ELISA.Human TNF alpha was diluted in serial 2-fold steps in Sample Diluent. Do not use this more

Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution
Standard Curve Range
23 - 1,500 pg/mL
5.0 pg/mL

Order Details

Human TNF-alpha ELISA Kit (Colorimetric) Summary

The assay detects both natural and recombinant Product. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human TNF alpha positive serum. There was no interference detected, namely not with TNF-R (60 kDa and 80 kDa).
Standard Curve Range
23 - 1,500 pg/mL
5.0 pg/mL
Assay Type
Sandwich ELISA
Spike Recovery
The recovery ranged from 76% to 115% with an overall mean recovery of 90%. Please see protocol for more specific Spike Recovery details.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)


Application Notes
The human TNF-alpha total ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human TNF-alpha

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.

Kit Components

  1. 1 vial (22 ul) Streptavidin-HRP
  2. 1 vial (2.2 ml) monoclonal Coating Antibody to human TNF-alpha total (100 ug/ml)
  3. 1 vial (55 ul) Biotin-Conjugate anti-human TNF-alpha total polyclonal antibody
  4. 1 vial human TNF-alpha total Standard protein lyophilized, 30 ng/ml upon reconstitution dilution
  5. 2 vials (50 ml) Sample Diluent

Alternate Names for Human TNF-alpha ELISA Kit (Colorimetric)

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Rb
Applications: WB, Flow, IHC-P, KO
Species: Hu
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
Species: Hu
Applications: Flow, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: ELISA

Publications for TNF-alpha ELISA Kit (NBP1-83741) (0)

There are no publications for TNF-alpha ELISA Kit (NBP1-83741).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TNF-alpha ELISA Kit (NBP1-83741) (0)

There are no reviews for TNF-alpha ELISA Kit (NBP1-83741). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF-alpha ELISA Kit (NBP1-83741). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Additional TNF-alpha Products

Bioinformatics Tool for TNF-alpha ELISA Kit (NBP1-83741)

Discover related pathways, diseases and genes to TNF-alpha ELISA Kit (NBP1-83741). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TNF-alpha ELISA Kit (NBP1-83741)

Discover more about diseases related to TNF-alpha ELISA Kit (NBP1-83741).

Pathways for TNF-alpha ELISA Kit (NBP1-83741)

View related products by pathway.

PTMs for TNF-alpha ELISA Kit (NBP1-83741)

Learn more about PTMs related to TNF-alpha ELISA Kit (NBP1-83741).

Research Areas for TNF-alpha ELISA Kit (NBP1-83741)

Find related products by research area.

Blogs on TNF-alpha.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human TNF-alpha ELISA Kit (Colorimetric) and receive a gift card or discount.


Gene Symbol TNF