TNF-alpha Antibody [Biotin]

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Product Details

Summary
Product Discontinued
View other related TNF-alpha Primary Antibodies

Order Details


    • Catalog Number
      NBP2-00399
    • Availability
      Product Discontinued

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TNF-alpha Antibody [Biotin] Summary

Immunogen
The immunogen for this antibody is TNF alpha polyclonal.
Clonality
Polyclonal
Host
Rabbit
Gene
TNF
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
Application Notes
The biotinylated rabbit anti-mouse/rat TNF-alpha polyclonal antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse or rat TNF-alpha in combination with the purified anti-mouse/rat TNF-alpha monoclonal antibody, clone TN3.19 (NBP1-43235) for capture and recombinant mouse TNF-alpha (NBP1-43197) or rat TNF-alpha (NBP1-43197) as the standard. A suitable range of concentrations of this biotinylated antibody for ELISA detection is 0.5 - 2.0 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml (mouse TNF-alpha) or 5000 - 30 pg/ml (rat TNF-alpha) should be included in each ELISA plate. Recognition of mouse TNF-alpha by this assay is not interfered with by 1000x excess soluble TNF-alpha receptor type I or II.

Reactivity Notes

Mouse, Rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TNF-alpha Antibody [Biotin]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

The affinity purified polyclonal rabbit antibody reacts with mouse and rat tumor necrosis factor-alpha. Mouse TNF alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4 T cells, and NK cells. A 26 kDa form is expressed as a membranebound molecule. TNF alpha is cytolytic and plays an important role in immune regulation. Bioactive TNF alpha exists as a homotrimer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for TNF-alpha Antibody (NBP2-00399). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TNF
Entrez