TNF-alpha Antibody (MAb11) [Biotin]


Sandwich ELISA: TNF-alpha Antibody (MAb11) [Biotin] [NB100-78162]

Product Details

Reactivity Hu, Po, Bb, PmSpecies Glossary
Applications ELISA, Flow, ICC/IF, S-ELISA
0.5 mg/ml

Order Details

Available Conjugates
Catalog# & Conjugate Size Price

TNF-alpha Antibody (MAb11) [Biotin] Summary

E. coli-expressed, recombinant human TNF- alpha
TNF- alpha (MAb11)
IgG1 Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Biotin (A=N/A, E=N/A)
PBS (pH 7.2)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


Application Notes
The purified MAb11 has been tested by blocking fluorochrome conjugated MAb11 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody ( le 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.
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NB100-78162 in the following applications:

Reactivity Notes

Human. Predicted cross-reactivity based on sequence identity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Sooty Mangabey, Swine (Pig, Porcine).

Alternate Names for TNF-alpha Antibody (MAb11) [Biotin]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


TNF- alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF- alpha. Monomeric human TNF- alpha is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF- alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF- alpha has also been described. TNF- alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF- alpha.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF-alpha Antibody (NB100-78162)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for TNF-alpha Antibody (NB100-78162). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies


Isotype Controls

Other Available Formats

FITC Labeled NB100-78163

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Contact Information

Product PDFs


Gene Symbol TNF

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