TNF-alpha Antibody (AP-MAB0856) Summary
Recombinant mouse TNF-alpha
This was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse TNF-alpha.
Protein A or G purified
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- Blocking/Neutralizing 50-200 molar excess
Neutralizing bioactivity of natural or recombinant TNF-alpha. Extremely low level of LPS (< 0.002EU/ug IgG)
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
A 0.2 um filtered solution in PBS - Azide free
Protein A or G purified
Alternate Names for TNF-alpha Antibody (AP-MAB0856)
- APC1 protein
- TNF, monocyte-derived
- TNFATNF, macrophage-derived
- TNFSF2TNF superfamily, member 2
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- Tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor
- tumor necrosis factor-alpha
TNF-alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-alpha. Monomeric mouse TNF-alpha is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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