TLR9 Antibody (M9.D6) [Biotin]


Flow Cytometry: TLR9 Antibody (M9.D6) [Biotin] [NBP1-43479] - Staining of fixed and permeabilized mouse TLR9-transfected 293 cells with 0.25 ug of Rat IgG2a Isotype Control Biotin (open histogram) or 0.25 ug of more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow
0.5 mg/ml

TLR9 Antibody (M9.D6) [Biotin] Summary

The immunogen for this antibody was Tolllike receptor 9.
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Biotin (A=N/A, E=N/A)
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


Application Notes
The M9.D6 antibody has been tested by intracellular flow cytometric analysis of fixed and permeabilized mouse TLR9 transfected cells. This can be used at less than or equal to 1.0 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Readout System

Reactivity Notes

This antibody is reactive to Mouse.

Alternate Names for TLR9 Antibody (M9.D6) [Biotin]

  • CD289 antigen
  • CD289
  • toll-like receptor 9


M9.D6 is generated against a peptide derived from the extracellular portion of mouse TLR9. Predominantly expressed as an intracellular protein, TLR9 is a ~115-120 kDa molecule which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9+ B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on RBC-lysed mouse splenocytes stained for intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Further studies are needed to determine the relationship between mRNA expression and protein detection.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies


Isotype Controls

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TLR9: For Whom the Cell Tolls
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Toll-like receptor 9 (TLR9) is a protein encoded by TLR9 gene in humans. It is also known as cluster of differentiation 289 (CD289) and is a member of TLR family. Proteins from TLR family are transmembrane proteins that expressed in both antigen-resen...  Read full blog post.

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Product PDFs


Gene Symbol TLR9

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