TLR7 Antibody

Images

 
Western Blot: TLR7 Antibody [NBP1-77205] - Daudi cell lysates with TLR7 antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.
Immunocytochemistry: TLR7 Antibody [NBP1-77205] - Immunocytochemistry staining of Daudi cells using TLR7 antibody at 2 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TLR7 Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the center of mouse TLR7. The immunogen is located within amino acids 420 - 470 of TLR7.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TLR7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunocytochemistry 2 ug/ml
  • Immunofluorescence
Application Notes
TLR7 antibody can be used for detection of TLR7 by Western blot at 0.5 to 2 mg/ml.
Theoretical MW
135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Agonist
Control Peptide
TLR7 Peptide (NBP1-77205PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TLR7 Antibody

  • TLR7
  • toll-like receptor 7

Background

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR7, like TLRs 3, 8, and 9, is localized in intracellular acidic compartments such as the phagolysosome and will recognize some single-stranded RNA viruses such as vesicular stomatitis virus (VSV) and influenza virus. Activation of TLR7 by VSV results in stimulation of the immune response including IFNalpha secretion, suggesting the importance of TLR7 in virus recognition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TLR7 Antibody (NBP1-77205) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TLR7 Antibody (NBP1-77205)

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Blogs on TLR7.

TLR9: For Whom the Cell Tolls
The Toll-like receptor 9 (TLR9) protein, also known as CD289, belongs to the family of Toll-like receptor (TLR) proteins which play a large role in pathogen recognition and the activation of innate immunity. Scientists using TLR9 antibodies have found...  Read full blog post.

TLR7 and Immune Response Regulation
Toll-like receptor 7 (TLR7) is a protein encoded by the TLR7 gene in humans and is a member of TLR family. TLRs controls host immune response against pathogens (e.g. viruses, bacteria and fungi) through recognition of pathogen-associated molecular pat...  Read full blog post.

TLR9 Antibodies in Immunity Research
Toll-like receptor 9 (TLR9) is a member of the toll-like receptor family that plays a key role in pathogen recognition and activation of innate immunity. Scientists using TLR9 antibodies have found the protein is highly conserved from Drosophila to hu...  Read full blog post.

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Bioinformatics

Gene Symbol TLR7
Entrez
Uniprot