TLR2 Antibody

Images

 
Western Blot: TLR2 Antibody [NB200-536] - Western blot analysis of TLR2 in A) human, B) mouse and C) rat liver lysate using NB200-536 at 5 ug/ml.
Flow Cytometry: TLR2 Antibody [NB200-536] - Cell surface flow cytometric analysis using TLR2 antibody. Mouse RAW cells were probed using 1 ug of TLR2 antibody (red) and 1 ug of isotype control antibody (green).
Western Blot: TLR2 Antibody [NB200-536] - Western blot analysis of mTLR2 in 15 ugs of Raw cell lysate using NB200-536 at 1:2000 dilution.

Product Details

Summary
Product Discontinued
View other related TLR2 Primary Antibodies

Order Details


    • Catalog Number
      NB200-536
    • Availability
      Product Discontinued

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TLR2 Antibody Summary

Immunogen
This antibody was developed against amino acids 375-390 of recombinant mouse TLR2 (AAF28345.1).
Localization
Type I membrane protein
Specificity
This immunogen sequence is 93% conserved between mouse and human, rat, and chimpanzee. The antibody will cross-react with human, rat, and chimpanzee TLR2. Users are encourage to use the sequence information and gene bank accession number (AAF28345.1) to assess potential cross-reactivity of the antibody to other species that they may be interested in. In RAW, a 86 kDa band is observed.
Clonality
Polyclonal
Host
Rabbit
Gene
TLR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 4-6 ug/ml
  • Flow Cytometry 1 ug/10^6 cells in 100 ul
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
TLR2 Lysate (NBL1-16952)
Publications
Read Publications using
NB200-536 in the following applications:

  • 2 publications
  • WB
    2 publications

Reactivity Notes

Mouse. Predicted cross-reactivity based on immunogen sequence identity: Human(93%), Rat(93%) and Chimpanzee(93%). Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TLR2 Antibody

  • CD282 antigen
  • CD282
  • TIL4CD282
  • TLR2
  • toll/interleukin 1 receptor-like 4
  • Toll/interleukin-1 receptor-like protein 4
  • toll-like receptor 2

Background

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up-regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, IHC, IHC-P
Supplier Logo

Publications for TLR2 Antibody (NB200-536)(4)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(2)
WB
(2)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TLR2 Antibody (NB200-536). (Showing 1 - 2 of 2 FAQs).

  1. The amino acids 375-390 of mouse TLR2 is the intracellular portion or the extracellular portion?
    • Amino acids 375-390 of the TLR2 protein correspond to the extracellular domain.
  2. Would the product TLR2 Antibody (NB200-536) be useful in the application of immunoprecipitation?
    • NB200-536 has not been validated for use in Immunoprecipitation. If you would be interested in testing this novel application, please take a look at our Innovators Reward Program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional TLR2 Products

Bioinformatics Tool for TLR2 Antibody (NB200-536)

Discover related pathways, diseases and genes to TLR2 Antibody (NB200-536). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for TLR2 Antibody (NB200-536)

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Pathways for TLR2 Antibody (NB200-536)

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Research Areas for TLR2 Antibody (NB200-536)

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Blogs on TLR2.

The role of STING/TMEM173 in gamma and encephalitis Herpes Simplex Virus (HSV)
Stimulator of interferon genes (STING), also known as TMEM173, promotes the production of the interferon’s IFN-alpha and IFN-beta.  STING possesses three functional domains: a cytoplasmic C-terminal tail, a central globular domain, and four N-...  Read full blog post.

TRIF/TICAM1 and mitochondrial dynamics in the innate immune response
TRIF, also known as toll like receptor adaptor molecule 1 or TICAM1, is known for its role in invading foreign pathogens as part of our innate immune response. TRIF/TICAM1 is a TIR-domain adaptor protein (toll/interleukin-1 receptor) that interacts...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

The role of TLR4 in breast cancer
Toll like receptors (TLRs) are highly conserved proteins that are first known for their role in pathogen recognition and immune response activation.  In order to elicit the necessary immune response in reaction to a foreign pathogen, TLRs trigger cy...  Read full blog post.

TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.

IRAK4: The "master IRAK" critical for initiating immune responses
IRAK4, also known as Interleukin-1 receptor-associated kinase 4, is a serine/threonine-protein kinase that plays a critical role in initiating innate and adaptive immune responses against foreign pathogens. It activates NF-kappaB in both Toll-like rec...  Read full blog post.

TLR1
TLR1 belongs to the Toll-like receptor (TLR) family, and is a key player in the recognition of pathogens as well as the activation of the innate immunity system. TLRs are highly conserved proteins with a high degree of structural and functional homolo...  Read full blog post.

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Bioinformatics

Gene Symbol TLR2
Entrez
Uniprot