Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line Summary
The TLR12 reporter line may be useful for TLR12 based functional studies. The functional activity of the NF-kB/SEAP reporter in the TLR12 stable cell line has been established and validated through PMA activation of the NF-kB pathway. Hence, the cell line may be useful for reseasrchers screening for putative TLR12 ligands activating the NF-kB pathway.
TLR12 expression in this cell line has been validated by Western blotting (Fig 1) and flow cytometry (Fig 2).
Contents: 3~4 x 10^6 cells
Biosafety Level: 2
The TLR12 reporter cell line is a stably transfected cell line which expresses full-length mouse Toll-like receptor 12 (TLR12) with an N-terminal HA tag and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element.
Adherent Morphology: Epithelial
NF-kB/SEAP - (SEAPorter™)
Secreted alkaline phosphatase (SEAP)
Packaging, Storage & Formulations
Store in gas phase of liquid nitrogen.
Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.
Details for Array
Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the stable cell line.
- Wash hands after handling the stable cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.
Alternate Names for Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line
- toll-like receptor 12
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed
for 1 year from date of receipt.
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