Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

Images

 
Western Blot: Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26288] - Analysis of TLR12 expression in the TLR12 reporter cell line using an HA antibody (20 ug total protein/lane). Legend. ...read more
Flow Cytometry: Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26288] - Expression of TLR12 in TLR12 reporter cell line was analyzed by flow cytometry using a FITC-conjugated TLR12 antibody ...read more
Ligand Activation: Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26288] - TLR12 reporter cells were plated in a 96-well plate at 5 x 10^4 cells per well. Cells were stimulated with various ...read more

Product Details

Summary
Applications WB, Flow
Host
HEK293

Order Details

Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line Summary

Description
The TLR12 reporter line may be useful for TLR12 based functional studies. The functional activity of the NF-kB/SEAP reporter in the TLR12 stable cell line has been established and validated through PMA activation of the NF-kB pathway. Hence, the cell line may be useful for reseasrchers screening for putative TLR12 ligands activating the NF-kB pathway. TLR12 expression in this cell line has been validated by Western blotting (Fig 1) and flow cytometry (Fig 2).

Contents: 3~4 x 10^6 cells
Biosafety Level: 2
Immunogen
The TLR12 reporter cell line is a stably transfected cell line which expresses full-length mouse Toll-like receptor 12 (TLR12) with an N-terminal HA tag and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element.
Target Species
Mouse
Specificity
TLR12
Selection Agent
Blasticidin
Growth Properties
Adherent Morphology: Epithelial
RCL Type
NF-kB/SEAP - (SEAPorter™)
Reporter Gene
Secreted alkaline phosphatase (SEAP)
Host
HEK293
Gene
TLR12

Applications/Dilutions

Packaging, Storage & Formulations

Storage
Store in gas phase of liquid nitrogen.
Reconstitution Instructions
Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for Array

Type
Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the stable cell line.
- Wash hands after handling the stable cell line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

  • Gm1365
  • TLR12
  • toll-like receptor 12

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for TLR12 Reporter Cell Line (NBP2-26288): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol TLR12
Entrez