Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

Images

 
Western Blot: Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26273] - Analysis of TLR10 expression in the TLR10 reporter cell line using an HA antibody (20 ug total protein/lane). Legend: ...read more
Flow Cytometry: Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26273] - Analysis of TLR10. Cell surface expression of TLR10 in TLR10 reporter cell line was analyzed by flow cytometry using a ...read more
Ligand Activation: Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26273] - TLR10 reporter cells were plated in a 96-well plate at 5 x 10^4 cells per well. Cells were stimulated with various ...read more

Product Details

Summary
Applications WB, Flow
Host
HEK293

Order Details

Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line Summary

Description
The TLR10 reporter line may be useful for TLR10-based functional studies. The functional activity of the NF-kB/SEAP reporter in the TLR10 stable cell line has been established and validated through PMA activation of the NF-kB pathway (Figure 1). Hence, the cell line may be useful for reseasrchers screening for putative TLR10 ligands activating the NF-kB pathway.

Any specific ligand for TLR10 is still unknown. However, TLR10 can indeed activate the NF-kB signaling pathway through direct association with the common Toll/IL-1 receptor domain adapter, MyD88, according to a recent study utilizing a chimeric CD4/TLR10 molecule. TLR10 expression in the TLR10/NF-kB/SEAPorter HEK 293 cell line has been validated by Western blotting (Fig. 2) and flow cytometry (Fig. 3).

Contents: 3~4 x 10^6 cells
Biosafety Level: 2
Immunogen
The TLR10/NF-kB/SEAPorter HEK 293 cell line is a stably transfected cell line which expresses full-length human Toll-like receptor 10 (TLR10) with an N-terminal HA tag and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element.
Target Species
Human
Specificity
TLR10/NF-kB/SEAP
Selection Agent
Blasticidin and G418.
Growth Properties
Adherent Morphology: Epithelial
RCL Type
NF-kB/SEAP - (SEAPorter™)
Reporter Gene
Secreted alkaline phosphatase (SEAP)
Host
HEK293
Gene
TLR10

Applications/Dilutions

Packaging, Storage & Formulations

Storage
Store in gas phase of liquid nitrogen.
Reconstitution Instructions
Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin + 0.5 mg/ml G418 (Geneticin).

Details for Array

Type
Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the TLR10 reporter line.
- Wash hands after handling the TLR10 reporter line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

  • CD290 antigen
  • CD290
  • MGC104967
  • MGC126398
  • MGC126399
  • TLR10
  • toll-like receptor 10

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol TLR10
Entrez