TLR1 Antibody (GD2.F4) [PE]


Flow Cytometry: TLR1 Antibody (GD2.F4) [PE] [NBP2-00253] - Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE (open histogram) or Anti-Human CD281 (TLR1) PE (filled histogram). more

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TLR1 Antibody (GD2.F4) [PE] Summary

The immunogen for this antibody is TLR1.
IgG1 Kappa
Protein A or G purified
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  • Flow Cytometry 1:10-1:1000
Application Notes
The GD2.F4 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 ul (1.0 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
Protein A or G purified

Alternate Names for TLR1 Antibody (GD2.F4) [PE]

  • CD281 antigen
  • CD281
  • DKFZp547I0610
  • DKFZp564I0682
  • KIAA0012MGC126311
  • MGC104956
  • MGC126312
  • rsc786
  • TIL
  • TIL. LPRS5
  • TLR1
  • Toll/interleukin-1 receptor-like protein
  • toll-like receptor 1


The GD2.F4 antibody reacts with human Toll-like receptor 1 (TLR1). TLR1 is expressed in low numbers on the surface of peripheral blood monocytes and even lower numbers on dendritic cells. A very high degree of donor variability has been reported for TLR1 expression. GD2.F4 mAb has been tested by flow cytometry, in parallel with a sample from the developer using cells from healthy donors. Donors tested confirmed low levels expression of TLR1 on PBMC (less than 1/4 log shift). While the ligand and exact function of TLR1 are not defined yet, it has been suggested that TLR1 may cooperate with TLR2 in response to certain antagonists. Despite low levels of surface expression as detected by flow cytometric analysis, TLRs are efficient signal transducing molecules in monocytes and dendritic cells. To date, at least ten members of the Toll family have been identified in huma. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for TLR1 Antibody (NBP2-00253). (Showing 1 - 1 of 1 FAQ).

  1. Could I please have the concentration of PE-TLR1 (clone GFD2.F4), product # NBP2-00253, lot # EO2240-1633?
    • The concentration of our product NBP2-00253 is 0.2mg/ml.

Secondary Antibodies


Isotype Controls

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Gene Symbol TLR1