TIM-3 Antibody (B8.2C12) [Biotin]


Flow Cytometry: TIM-3 Antibody (8B.2C12) [Biotin] [NBP1-43537] - Staining of TIM-3 transfected cells with Anti-Mouse TIM3 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for ...read more

Product Details

Reactivity MuSpecies Glossary
Applications Flow
0.5 mg/ml

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Available Conjugates
Available Formulations
Catalog# & Formulation Size Price

TIM-3 Antibody (B8.2C12) [Biotin] Summary

Recombinant mouse TIM-3 protein
IgG1 Kappa
Protein A or G purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Biotin (A=N/A, E=N/A)
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified


  • Flow Cytometry 1:10-1:1000
Application Notes
The 8B.2C12 antibody has been tested by flow cytometric analysis of mouse splenocytes and mouse Tim-3 transfected cells and can be used at less than or equal to 0.06 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Readout System

Reactivity Notes


Alternate Names for TIM-3 Antibody (B8.2C12) [Biotin]

  • CD366
  • FLJ14428
  • HAVCR2
  • HAVcr-2
  • hepatitis A virus cellular receptor 2
  • kidney injury molecule-3
  • T cell immunoglobulin mucin 3
  • T cell immunoglobulin mucin-3
  • T-cell immunoglobulin and mucin domain-containing protein 3
  • TIM 3
  • TIM3 T-cell membrane protein 3
  • TIM3
  • Tim-3
  • TIMD-3
  • TIMD3KIM-3


The 8B.2C12 monoclonal antibody reacts with mouse Tim-3, a Th1-specific cell surface protein. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. Tim-3 is expressed selectively by differentiated CD4+Th1 and CD8+Tc1 cells, but is absent on CD4+Th2 and CD8+Tc2 cells. Other hematopoietic cell types, including nave T cells, B cells, macrophages and dendritic cells, do not express Tim-3, at least at the protein level. Tim-3 expression is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, Tim-3 was shown to be expressed on most CD4+ and CD8+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4+ cells in the periphery expressed Tim-3 after immunization. In this model, in vivo administration of 8B.2C12 resulted in a hyperacute and atypical disease phenotype. It is postulated that the engagement of Tim-3 during T cell activation results in the expansion and activation of macrophages and increased severity of an autoimmune disease. The Tim gene family may have an important role in the regulation of autoimmunity and allergies. The 8B.2C12 antibody binds to the Tim-3 BALB/c allele. Reactivity to the C57/Bl6 is significantly weaker than BALB/c.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

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Blogs on TIM-3.

TIM-3, a critical immune checkpoint in HIV research
CD4+ T-helper cells (Th) are the white blood lymphocytes expressing surface glycoprotein antigen CD4. These T-helper cells play an important role in the adaptive immune system by releasing T cell cytokines that help other immune cells to suppress o...  Read full blog post.

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Product PDFs


Gene Symbol HAVCR2

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