TIM-3 Antibody (B8.2C12) [Biotin]

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Flow Cytometry: TIM-3 Antibody (8B.2C12) [Biotin] [NBP1-43537] - Staining of TIM-3 transfected cells with Anti-Mouse TIM3 PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-43537
    • Availability
      Product Discontinued

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TIM-3 Antibody (B8.2C12) [Biotin] Summary

Immunogen
Recombinant mouse TIM-3 protein
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Gene
HAVCR2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The 8B.2C12 antibody has been tested by flow cytometric analysis of mouse splenocytes and mouse Tim-3 transfected cells and can be used at less than or equal to 0.06 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Readout System

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for TIM-3 Antibody (B8.2C12) [Biotin]

  • CD366
  • FLJ14428
  • HAVCR2
  • HAVcr-2
  • hepatitis A virus cellular receptor 2
  • kidney injury molecule-3
  • T cell immunoglobulin mucin 3
  • T cell immunoglobulin mucin-3
  • T-cell immunoglobulin and mucin domain-containing protein 3
  • TIM 3
  • TIM3 T-cell membrane protein 3
  • TIM3
  • TIM-3
  • TIMD-3
  • TIMD3KIM-3

Background

The 8B.2C12 monoclonal antibody reacts with mouse Tim-3, a Th1-specific cell surface protein. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. Tim-3 is expressed selectively by differentiated CD4+Th1 and CD8+Tc1 cells, but is absent on CD4+Th2 and CD8+Tc2 cells. Other hematopoietic cell types, including nave T cells, B cells, macrophages and dendritic cells, do not express Tim-3, at least at the protein level. Tim-3 expression is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, Tim-3 was shown to be expressed on most CD4+ and CD8+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4+ cells in the periphery expressed Tim-3 after immunization. In this model, in vivo administration of 8B.2C12 resulted in a hyperacute and atypical disease phenotype. It is postulated that the engagement of Tim-3 during T cell activation results in the expansion and activation of macrophages and increased severity of an autoimmune disease. The Tim gene family may have an important role in the regulation of autoimmunity and allergies. The 8B.2C12 antibody binds to the Tim-3 BALB/c allele. Reactivity to the C57/Bl6 is significantly weaker than BALB/c.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Diseases for TIM-3 Antibody (NBP1-43537)

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PTMs for TIM-3 Antibody (NBP1-43537)

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Research Areas for TIM-3 Antibody (NBP1-43537)

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Blogs on TIM-3.

TIM-3, a critical immune checkpoint in HIV research
CD4+ T-helper cells (Th) are the white blood lymphocytes expressing surface glycoprotein antigen CD4. These T-helper cells play an important role in the adaptive immune system by releasing T cell cytokines that help other immune cells to suppress o...  Read full blog post.

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Bioinformatics

Gene Symbol HAVCR2
Entrez