Tie-2 Antibody (2D2)

Images

 
Western Blot: Tie-2 Antibody (2D2) [H00007010-M05] - Analysis of TEK expression in U-2 OS (Cat # L022V1).
Sandwich ELISA: Tie-2 Antibody (2D2) [H00007010-M05] - Detection limit for recombinant GST tagged TEK is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      H00007010-M05
    • Availability
      Product Discontinued

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Tie-2 Antibody (2D2) Summary

Immunogen
TEK (AAH35514, 701 a.a. - 800 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFSHELVTLPESQAPADLGGGKMLLIAILGSAGMTCLTVLLAFLIILQLKRANVQRRMAQAFQNVREEPAVQFNSGTLALNRKVKNNPDPTIYPVLDWND
Specificity
TEK - TEK tyrosine kinase, endothelial (venous malformations, multiple cutaneous and mucosal)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TEK
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Tie-2 Antibody (2D2)

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TEK
Entrez
OMIM
Uniprot