Recombinant Human Thioredoxin Protein (NBP1-44488)

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SDS-Page: Thioredoxin Protein [NBP1-44488] - TXN, 13.9 kDa (125aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human Thioredoxin Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-105 of Human Thioredoxin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV

Preparation
Method
E.coli
Details of Functionality
Specific activity is >100 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Protein/Peptide Type
Biologically Active Protein
Gene
TXN
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Application Notes
Assay
1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA.
2. Add 4ug of recombinant His-TXN into reaction cocktail and mix immediately.
3. Incubate at 25C for 20 minutes.
4. Record the increase in A650nm for 15 minutes
Theoretical MW
13.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

This is a Human protein

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
Phosphate Buffered Saline pH7.4
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Thioredoxin Protein

  • ADF
  • ATL-derived factor
  • MGC61975
  • SASP
  • Surface-associated sulphydryl protein
  • thioredoxin
  • TRDX
  • Trx
  • TRX1
  • TRXDKFZp686B1993

Background

SQSTM1 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and play a role in titin/TTN downstream signaling in muscle cells. This protein also may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of the sequestosome 1 protein commonly cause Paget's disease since the UBA is necessary for aggregate sequestration and cell survival. Recombinant SQSTM1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol TXN