Thioredoxin Recombinant Protein

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SDS-Page: Thioredoxin Protein [NBP1-44488] - TXN, 13.9 kDa (125aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1.0 mg/ml

Order Details

Thioredoxin Recombinant Protein Summary

Description
A recombinant protein with a His tag and corresponding to amino acids 1 - 105 of TXN.

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV

Preparation
Method
E.coli
Details of Functionality
Specific activity is 7-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Gene
TXN
Purity
>95% pure by SDS-PAGE

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Phosphate Buffered Saline pH7.4
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Application Notes
Assay
1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA.
2. Add 4ug of recombinant His-TXN into reaction cocktail and mix immediately.
3. Incubate at 25C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

Reactivity Notes

This is a Human protein

Notes

The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 13.9 kDa (125aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Alternate Names for Thioredoxin Recombinant Protein

  • ADF
  • ATL-derived factor
  • MGC61975
  • SASP
  • Surface-associated sulphydryl protein
  • thioredoxin
  • TRDX
  • Trx
  • TRX1
  • TRXDKFZp686B1993

Background

SQSTM1 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 and play a role in titin/TTN downstream signaling in muscle cells. This protein also may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of the sequestosome 1 protein commonly cause Paget's disease since the UBA is necessary for aggregate sequestration and cell survival. Recombinant SQSTM1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol TXN

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