Th-POK Antibody

Images

 
Immunohistochemistry: Th-POK Antibody [NBP1-77810] - Tissue: small intestine Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized. Used at a 1:100 dilution.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-77810
    • Availability
      Product Discontinued

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Th-POK Antibody Summary

Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal sequence of mouse cKrox protein.
Specificity
This antibody is directed against mouse cKrox. A BLAST analysis was used to suggest cross-reactivity with c-Krox from mouse, human and rat based on a 100% identity with the immunizing sequence.
Clonality
Polyclonal
Host
Rabbit
Gene
ZBTB7B
Purity
Delipidation and Defibrination
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:2500-1:10000
  • Immunohistochemistry 1.25-2.5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Expect a band approximately 80 kDa in size corresponding - cKrox by western blotting in the appropriate cell lysate or extract. Lysates from mouse thymocytes are recommended for use as positive controls.

Reactivity Notes

Reactive in mouse, human, rat

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Delipidation and Defibrination

Alternate Names for Th-POK Antibody

  • c-Krox
  • hcKroxc-Krox
  • Krueppel-related zinc finger protein cKrox
  • kruppel-related zinc finger protein hcKrox
  • T-helper-inducing POZ/Krueppel-like factor
  • ThPOK
  • Th-POK
  • ZBTB15
  • ZBTB15zinc finger protein 67 homolog (mouse)
  • ZBTB7B
  • ZFP67
  • zfp-67
  • zinc finger and BTB domain containing 15
  • zinc finger and BTB domain containing 7B
  • Zinc finger and BTB domain-containing protein 15
  • zinc finger and BTB domain-containing protein 7B
  • Zinc finger protein 67 homolog
  • Zinc finger protein 857B
  • Zinc finger protein Th-POK
  • ZNF857B
  • ZNF857BDKFZp686G01254

Background

cKrox (also known as Zfp67 or ThPOK) functions as a transcription regulator that acts as a key modulator of lineage commitment for immature T-cell precursors. cKrox is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. cKrox is a known transcriptional repressor of the collagen COL1A1 and COL1A2 genes and may also function as a repressor of fibronectin and possibly other extracellular matrix genes. cKrox is located within the nucleus and is expressed in multiple tissues and organs including skin, thymus and peripheral T-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Th-POK Antibody (NBP1-77810). (Showing 1 - 1 of 1 FAQ).

  1. Can you send me the validation data for the IHC-P or put it on the website and what ab dilution or amount is required for that application? Are you sending me in house validation images, or putting them on the website?
    • We recommend a concentration of 1.25-2.5 ug/ml for use of NBP1-77810 in IHC on paraffin-embedded tissues. If we have images, they will be posted on the website. This may have been validated for IHC-P use by customer feedback so images may not be available. It is still guaranteed for all applications and species listed on our datasheet.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ZBTB7B
Entrez
Uniprot