TGN46 Antibody

Product Discontinued

TGN46 Antibody Summary

• Immunogen: Antisera to TGN46 were raised by repeated immunisations of sheep with highly purified recombinant human TGN46
• Marker: TGN Marker
• Specificity: TGN46 - Golgi Complex Marker
• Clonality: Polyclonal
• Host: Sheep
• Gene: TGOLN2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Application Notes: Immunofluorescence Fixation with 3% paraformaldehyde or methanol is recommended and incubation for 60 min at room temperature in blocking buffer (0.1% gelatin in TBS/triton) Western blotting in 5% milk/TBS (+ 0.1% Tween-20) and incubation for 60 min at room temperature. Immunohistology: Fixation with 3% paraformaldehyde or methanol/acetone is recommended
• Control:

  TGN38 Overexpression Lysate

Reactivity Notes

Reacts with Primate, Human and Rat. Antibody reactivity and working conditions vary between species

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for TGN46 Antibody

• TGN 46
• TGN 48
• TGN 51
• TGN38 homolog
• TGN38
• TGN-38
• TGN46
• TGN-46
• TGN48
• TGN51
• TGOLN 2
• TGOLN2
• trans golgi network 38
• trans golgi network 46
• Trans Golgi network integral membrane protein 2 [Precursor]
• Trans Golgi network integral membrane protein 2
• Trans Golgi network protein (46 48 51kD isoforms)
• Trans golgi network protein 2
• Trans Golgi network protein TGN51
• Trans-Golgi network integral membrane protein 2
• Trans-Golgi network protein 2
• Trans-Golgi network protein TGN51
• TTGN 2
• TTGN2

Background

Sheep anti-human TGN46 recognises a glycoprotein designated TGN46 that is localised primarily in the Trans-Golgi network. TGN46 has been reported as being the best available marker for human trans-Golgi network

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN46 Antibody (NB110-60520)(13)

Publications using NB110-60520

• Uemura T, Sawada N, Sakaba T et al. Intracellular localization of GGA accessory protein p56 in cell lines and central nervous system neurons Biomed. Res. 2018-08-14 [PMID: 30101838] (ICC/IF, Human)
• Gonzalez C, Canovas J, Fresno J et al. Axons provide the secretory machinery for trafficking of voltage-gated sodium channels in peripheral nerve Proc. Natl. Acad. Sci. U.S.A. 2016-02-16 [PMID: 26839409]
• Gagnon H, Refaie S, Gagnon S et al. Proprotein Convertase 1/3 (PC1/3) in the Rat Alveolar Macrophage Cell Line NR8383: Localization, Trafficking and Effects on Cytokine Secretion. PLoS One 2013-04-24 [PMID: 23637853] (ICC/IF, Rat)
• Brodeur J, Theriault C, Lessard-Beaudoin M et al. LDLR-related protein 10 (LRP10) regulates amyloid precursor protein (APP) trafficking and processing: evidence for a role in Alzheimer's disease Mol Neurodegener 2012-06-26 [PMID: 22734645] (ICC/IF, Human)
• Kosaka T, Kuroha Y, Tada M et al. A fatal neuromuscular disease in an adult patient after poliomyelitis in early childhood: Consideration of the pathology of post-polio syndrome Neuropathology 2012-06-04 [PMID: 22672529] (IF/IHC, Human)
• Yi L, Donsante A, Kennerson ML, Mercer JF, Garbern JY, Kaler SG. Altered intracellular localization and valosin-containing protein (p97 VCP) interaction underlie ATP7A-related distal motor neuropathy. Hum Mol Genet;21(8):1794-807. 2012-04-15 [PMID: 22210628] (ICC/IF, Mouse)
• Drakesmith H et al. HIV-1 Nef down-regulates the hemochromatosis protein HFE, manipulating cellular iron homeostasis. PNAS; 102: 11017-11022. 2005-01-01 [PMID: 16043695]
• Mills IG et al. Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors. J Cell Biol; 70: 191-200. 2005-01-01 [PMID: 16027218]
• Vuillier F et al. Lower levels of surface B-cell-receptor expression in chronic lymphocytic leukemia are associated with glycosylation folding defects of the mu CD79a chains. Blood; 105: 2933-2940. 2005-01-01 [PMID: 15591116]
• Linstedt AD et al. A Cycling cis-Golgi Protein Mediates Endosome-to-Golgi Traffic. Mol Biol Cell; 15: 4798-4806. 2004-01-01 [PMID: 15331763]
• Marullo S et al. Homodimerization of the {beta}2-Adrenergic Receptor as a Prerequisite for Cell Surface Targeting. J Biol Chem; 279: 33390-33397. 2004-01-01 [PMID: 15155738]
• Prescott AR et al. Distinct compartmentalization of TGN46 beta 1, 4-galactosyltransferase in HeLa cells. Eur J Cell Biol; 72: 238-246. 1997-01-01 [PMID: 9084986]
• Ponnambalam S et al. Primate homologues of rat TGN38: primary structure, expression functional implications. J Cell Sci; 109: 675-685. 1996-01-01 [PMID: 8907712]

Bioinformatics

• Gene Symbol: TGOLN2
• Entrez:
  • 10618(Human)
• Uniprot:
  • O43493(Human)