TGN46 Antibody

Product Discontinued

TGN46 Antibody Summary

• Immunogen: Antisera to TGN46 were raised by repeated immunizations of rabbits with highly purified recombinant human THN46.
• Marker: TGN Marker
• Specificity: This is specific for TGN46.TGN46 is a heavily glycosylated protein of around 110-120kDa.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGOLN2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunomicroscopy
  • Western Blot 1:500-1000
• Application Notes: This antibody is useful for Western Blot, Immunofluorescence, and immunoelectron microscopy. For Immunofluorescene fixation with 3% paraformaldehyde or methanol is recommended, and incubation for 60 minutes at room temperature in blocking buffer (0.1% gelatin in TBS/Triton). For Western Blot it is recommended in 5% milk/TBS (+0.1% Tween-20) and incubation for 60 minutes at room temperature.
• Control:

  TGN38 Overexpression Lysate

Reactivity Notes

This antibody reacts with the human and primate protein. Antibody reactivity and working conditions vary between species. Other species have not yet been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for TGN46 Antibody

• TGN 46
• TGN 48
• TGN 51
• TGN38 homolog
• TGN38
• TGN-38
• TGN46
• TGN-46
• TGN48
• TGN51
• TGOLN 2
• TGOLN2
• trans golgi network 38
• trans golgi network 46
• Trans Golgi network integral membrane protein 2 [Precursor]
• Trans Golgi network integral membrane protein 2
• Trans Golgi network protein (46 48 51kD isoforms)
• Trans golgi network protein 2
• Trans Golgi network protein TGN51
• Trans-Golgi network integral membrane protein 2
• Trans-Golgi network protein 2
• Trans-Golgi network protein TGN51
• TTGN 2
• TTGN2

Background

The Trans-Golgi network integral membrane protein 2 (TGOLN2) was designated TGN46 based on the predicted molecular mass (46kDa). However, TGN46 is a heavily glycosylated protein, so its molecular weight is often detected at a 110-120kDa. The TGN46 protein is widely expressed. It may be involved in regulating membrane traffic to and from trans-Golgi network, and has been reported as the best available marker for human trans-Golgi network. TGN46 contains a luminal domain, a membrane-spanning domain, and a cytoplasmic domain. The membrane-spanning and cytoplasmic domains contain the retention and retrieval signals, respectively, for localization in the TGN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGN46 Antibody (NB110-40769)(8)

Publications using NB110-40769

• Dorobantu CM, Albulescu L, Harak C et al. Modulation of the Host Lipid Landscape to Promote RNA Virus Replication: The Picornavirus Encephalomyocarditis Virus Converges on the Pathway Used by Hepatitis C Virus. PLoS Pathog. 2015-09-01 [PMID: 26406250] (ICC/IF, Human)
• Binda C, Genier S, Cartier A et al. A G protein-coupled receptor and the intracellular synthase of its agonist functionally cooperate. J Cell Biol. 2014-02-04 [PMID: 24493589] (ICC/IF, Human)
  
  Details:
  TGN46 antibody used for ICC-IF on HEK293 cells transiently transfected with pcDNA3-Flag-DP1 or pcDNA3-L-PGDS-HA (Figure 2).
• Oner SS, Vural A, Lanier SM. Translocation of Activator of G-protein Signaling 3 to the Golgi apparatus in response to receptor activation and its effect on the trans-Golgi network. J Biol Chem 2013-06-14 [PMID: 23770668] (ICC/IF, Human)
• Frijns E, Kuikman I, Litjens S, Raspe M, Jalink K, Ports M, Wilhelmsen K, Sonnenberg A. Phosphorylation of threonine 1736 in the C-terminal tail of integrin ?4 contributes to hemidesmosome disassembly. Mol Biol Cell23(8):1475-85. 2012-04-01 [PMID: 22357621]
• Subathra M, Qureshi A, Luberto C. Sphingomyelin synthases regulate protein trafficking and secretion. PLoS ONE;6(9). 2011-01-01 [PMID: 21980337]
• Vire B, David A, Wiestner A. TOSO, the Fcmicro receptor, is highly expressed on chronic lymphocytic leukemia B cells, internalizes upon IgM binding, shuttles to the lysosome, and is downregulated in response to TLR activation J Immunol 2011-10-15 [PMID: 21908732] (ICC/IF, Human)
• Bacac M, Fusco C, Planche A et al. Securin and separase modulate membrane traffic by affecting endosomal acidification Traffic 2011-05-01 [PMID: 21272169] (WB, Human)
• Clarke CJ, Guthrie JM, Hannun YA. Regulation of Neutral Sphingomyelinase-2 (nSMase2) by Tumor Necrosis Factor-{alpha} Involves Protein Kinase C-{delta} in Lung Epithelial Cells. Mol Pharmacol;74(4):1022-1032. 2008-01-01 [PMID: 18653803]

Bioinformatics

• Gene Symbol: TGOLN2
• Entrez:
  • 10618(Human)