TGF-beta 1 Antibody

Product Discontinued

TGF-beta 1 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human TGF beta1. The immunogen region is: C-terminal.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGFB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 23531149).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TGF-beta 1 Antibody

• CEDLAP
• DPD1
• latency-associated peptide
• TGF beta
• TGF beta1
• TGFB
• TGFB1
• TGF-beta 1 protein
• TGFbeta 1
• TGF-beta 1
• TGFbeta
• TGF-beta-1
• transforming growth factor beta-1
• transforming growth factor, beta 1

Background

Transforming growth factor betas (TGFbetas) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFalpha. It is now realized that TGFbetas mediate many cell-cell interactions that occur during embryonic development. Three TGFbetas have been identified in mammals. TGFbeta1, TGFbeta2 and TGFbeta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFbeta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFbeta3 protein has approximately 80% identity to the mature region of both TGFbeta1 and TGFbeta2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TGF-beta 1 Antibody (NBP1-67698)(5)

Publications using NBP1-67698

• HusSain SM, Reed LF, Krasnick BA et al. IL23 and TGF-b diminish macrophage associated metastasis in pancreatic carcinoma. Sci Rep 2018-04-11 [PMID: 29643359] (IHC-P, Human)
• Li SS, He AL, Deng ZY, Liu QF. Ginsenoside-Rg1 Protects against Renal Fibrosis by Regulating the Klotho/TGF-b1/Smad Signaling Pathway in Rats with Obstructive Nephropathy. Biol. Pharm. Bull. 2018-04-03 [PMID: 29607931] (Rat)
• Wang H, Lewsadder M, Dorn E et al. RanBP9 overexpression reduces dendritic arbor and spine density. Neuroscience 2014-04-18 [PMID: 24486966] (WB)
• Palavicini JP, Wang H, Bianchi E et al. RanBP9 aggravates synaptic damage in the mouse brain and is inversely correlated to spinophilin levels in Alzheimer's brain synaptosomes. Cell Death Dis 2013-06-13 [PMID: 23764848] (WB, Mouse, Human)
• Hayes CD, Dey D, Palavicini JP et al. Striking reduction of amyloid plaque burden in an Alzheimer's mouse model after chronic administration of carmustine BMC Med 2013-03-26 [PMID: 23531149] (WB, Hamster)

Reviews for TGF-beta 1 Antibody (NBP1-67698) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Other.

reviewed by: Verified Customer WB Mouse 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23764848
• Species: Mouse

reviewed by: Verified Customer WB Other 12/23/2014

Summary

• Application: Western Blot
• Sample Tested: See PMID 23531149
• Species: Other

Bioinformatics

• Gene Symbol: TGFB1
• Entrez:
  • 7040(Human)
• OMIM:
  • 131300(Human)
• Uniprot:
  • P01137(Human)