TFIIE beta Antibody - BSA Free

Immunoprecipitation:TFIIE beta AntibodyNBP3-28990] -Detection of human GTF2E2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GTF2E2 antibody NBP3-28990 (lot NBP3-28990-1) used for IP at 6 µg per reaction. GTF2E2 was also immunoprecipitated by rabbit anti-GTF2E2 antibody. For blotting immunoprecipitated GTF2E2, NBP3-28990 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TFIIE beta Antibody - BSA Free Summary

• Immunogen: Between 241 and 291
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2E2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for TFIIE beta Antibody - BSA Free

• FE
• General transcription factor IIE subunit 2
• general transcription factor IIE, polypeptide 2 (beta subunit, 34kD)
• general transcription factor IIE, polypeptide 2, beta 34kDa
• TF2E2TFIIE-beta
• TFIIE-B
• transcription initiation factor IIE subunit beta

Background

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase andDNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2E2