TCR V beta 8.3 Antibody (B21.14) [FITC]


Flow Cytometry: TCR V beta 8.3 Antibody (B21.14) [FITC] [NBP2-00132] - Staining of C57BL/6 lymph node cells with Anti-Mouse CD4 APC and Anti-Mouse CD8 APC and 0.25 ug of Rat IgG1 kappa Isotype Control FITC (left) or more

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TCR V beta 8.3 Antibody (B21.14) [FITC] Summary

The immunogen for this antibody is TCR V beta 8.3.
IgG1 Kappa
Protein A or G purified
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  • Flow Cytometry 1:10-1:1000
Application Notes
This B21.14 antibody has been tested by flow cytometric analysis of mouse splenocytes and lymph node cells. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
0.09% Sodium Azide
0.5 mg/ml
Protein A or G purified

Alternate Names for TCR V beta 8.3 Antibody (B21.14) [FITC]

  • T cell receptor beta locus
  • T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster
  • T-cell receptor, beta cluster
  • TCRB
  • TRB


The B21.14 monoclonal antibody reacts with the mouse T-cell receptor (TCR) V-alpha 8.3 chain in all mouse strains tested. Specifically, the epitope of B21.14 has been mapped to three amino acids which are unique to V-alpha 8.3, but are not present in the other V-alpha 8 subfamily members.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol TRB