TCR beta Antibody (H57-597)

Images

 
Flow Cytometry: TCR beta Antibody (H57-597) [NBP1-30415] - Analysis of PE/Cy5 conjugate of NBP1-30415. Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.125 ug of Anti-Mouse ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
H57-597
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Concentration
1.0 mg/ml

TCR beta Antibody (H57-597) Summary

Immunogen
Affinity purified TCR from DO-11.10 T cell hybridoma
Specificity
The Armenian hamster monoclonal antibody H57-597 reacts specifically with beta subunit of TCR alpha/beta. This antibody does not crossreact with TCR gamma/delta type and has been used as TCR alpha/beta phenotypic marker.
Isotype
IgG
Clonality
Monoclonal
Host
Armenian Hamster
Gene
Tcrb
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/ml 1 million cells
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Functional Application: In vitro T cell stimulation; In vivo T cell depletion

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TCR beta Antibody (H57-597)

  • T cell receptor beta chain
  • TCRB
  • TCRbeta
  • Tib

Background

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for TCR beta Antibody (NBP1-30415) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-30415AF405
Alexa Fluor 488 Labeled NBP1-30415AF488
Alexa Fluor 647 Labeled NBP1-30415AF647
Alexa Fluor 700 Labeled NBP1-30415AF700
Allophycocyanin Labeled NBP1-30415APC
Biotin Labeled NBP1-28062
DyLight 350 Labeled NBP1-30415UV
DyLight 405 Labeled NBP1-30415V
DyLight 488 Labeled NBP1-30415G
DyLight 680 Labeled NBP1-30415FR
DyLight 755 Labeled NBP1-30415IR
PE Labeled NBP1-30415PE
PerCP Labeled NBP1-30415PCP

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Bioinformatics

Gene Symbol Tcrb