Human Talin1 ELISA Kit (Colorimetric)

ELISA: Human Talin1 ELISA Kit (Colorimetric) [NBP3-18044] - Samples were spiked with high concentrations of Human Talin1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Talin1 ELISA Kit (Colorimetric) [NBP3-18044] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 3.13-200 pg/mL
• Sensitivity: 1.88 pg/mL

Human Talin1 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 3.13-200 pg/mL
• Sensitivity: 1.88 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 5.79
• Intra-Assay: %CV < 5.04
• Spike Recovery: 92-111%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TLN1

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Talin1 ELISA Kit (Colorimetric)

• ILWEQ
• KIAA1027
• talin 1
• Talin1
• talin-1
• TLN
• TLN1

Background

Talin 1 encodes a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Talin1 ELISA Kit (NBP3-18044). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TLN1