Talin1 [p Ser425] Antibody

Western Blot: Talin1 [p Ser425] Antibody [NBP3-23437] - Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin1 (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin1 (Ser-425) (lanes 3 & 4) antibodies.

• Reactivity: Hu, Mu, Rt, Ch, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Talin1 [p Ser425] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-Talin1 (Ser-425) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding Ser-425 in human Talin1. This sequence is conserved in rat, mouse, and chicken Talin1, as well as in Talin2 (Ser-428). [UniProt# Q9Y490]
• Modification: p Ser425
• Specificity: The antibody detects a 240 kDa protein corresponding to the molecular mass of Talin1 on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TLN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Western Blot 1:1000
• Theoretical MW: 240 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Talin1 [p Ser425] Antibody

• ILWEQ
• KIAA1027
• talin 1
• Talin1
• talin-1
• TLN
• TLN1

Background

Talin 1 encodes a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLN1