Talin1 Antibody

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Western Blot: Talin 1 Antibody [NBP1-51979] - Western blot analysis of Talin 1 in Human Peripheral Mononucleocytes lysate (35ug protein in RIPA buffer) using NBP1-51979 at 1ug/ml. Primary incubation was 1 hour. Detected ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Talin1 Antibody Summary

Immunogen
Peptide with sequence C-QEALHTQMLTAVQE corresponding to internal region according to NP_006280.3.
Epitope
C-QEALHTQMLTAVQE
Specificity
This antibody is not expected to cross-react with the highly similar protein Talin 2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TLN1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: A >250 kDa band observed in human peripheral mononucleocyte lysates (calculated MW of 270 kDa band according to NP_006280.3). An additional band of unknown identity was also consistently observed at 110 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-51979.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Talin1 Antibody

  • ILWEQ
  • KIAA1027
  • talin 1
  • Talin1
  • talin-1
  • TLN
  • TLN1

Background

This gene encodes a cytoskeletal protein which is concentrated in areas of cell-substratum and cell-cell contacts. This protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Talin1 Antibody (NBP1-51979)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol TLN1
Entrez
Uniprot