TACC2 Overexpression Lysate (Native)

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Western Blot: TACC2 Lysate [NBL1-16670] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TACC2

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TACC2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215999 and based on accession number NM_006997. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens transforming, acidic coiled-coil containing protein 2 (TACC2), transcript variant 4, mRNA.
Gene
TACC2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
111.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TACC2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TACC2 Overexpression Lysate (Native)

  • anti zuai-1
  • AZU-1ECTACC
  • EC 2.4.2.10
  • EC 4.3.2.1
  • EC 6.3.5.2
  • transforming acidic coiled-coil-containing protein 2
  • transforming, acidic coiled-coil containing protein 2
  • Zuai-1

Background

Transforming acidic coiled-coil proteins are a conserved family of centrosome- and microtubule-interacting proteins that are implicated in cancer. This gene encodes a protein that concentrates at centrosomes throughout the cell cycle. This gene lies within a chromosomal region associated with tumorigenesis. Expression of this gene is induced by erythropoietin and is thought to affect the progression of breast tumors. Several transcript variants encoding different isoforms have been found for this gene. Transcript Variant: This variant (4) uses a different segment for its 5' UTR and coding region when compared to variant 1. The resulting protein (isoform d) has a shorter and distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TACC2 Lysate (NBL1-16670) (0)

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Video Protocols

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Bioinformatics

Gene Symbol TACC2