Western Blot: Synuclein-alpha Antibody [NBP2-25146] - Crude extract of rat brain, showing strong band at 15 kDa.
Immunocytochemistry/ Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Mixed rat neuron-glial cultures stained with NBP2-25146, polyclonal antibody to alpha-Synuclein (red) and monoclonal antibody to MAP2 ...read more
Immunocytochemistry/ Immunofluorescence: Synuclein-alpha Antibody [NBP2-25146] - Human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken-594 ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
10mM Sodium Azide
Immunogen affinity purified
This alpha-Synuclein antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at ~15 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 4 using NBP2-25146 in the following application:
synuclein, alpha (non A4 component of amyloid precursor)
Alpha-Synuclein is a member of the synuclein family, the other two proteins being beta and gamma synuclein. Alpha-Synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo. Direct homologues of alpha-Synuclein are found in all vertebrates. Later work connected alpha-Synuclein with human brain pathology, when a protein originally identified as a component of NAC, the "Non-Amyloid beta Component of Alzheimer's disease amyloid" proved to be a peptide derived from alpha-Synuclein. The alpha-Synuclein protein is therefore sometimes known as NAC precursor or NACP. Further work showed that alpha-Synuclein is a major component of the Lewy bodies of Parkinson's disease and point mutations of alpha-Synuclein proved to be causative of some forms of familial Parkinson's disease. However, despite being discovered as a component of amyloid preparations, alpha-Synuclein is apparently not a major component of the senile plaques of Alzheimer's disease. Early onset Parkinson's disease may be caused by a duplication or triplication of one of the alpha-Synuclein genes. Alpha-Synuclein is also found in the Lewy bodies of patients with diffuse Lewy body disease and inclusions in glial cells in the brains of patients with multiple system atrophy (MSA) and amyotrophic lateral sclerosis (ALS). Alpha-Synuclein is heavily expressed in brain and appears to be localized primarily to presynaptic regions, though not with a typical synaptic vesicle distribution pattern. The synuclein proteins appear to have little 3D structure in solution, and probably belong to the family of "intrinsically unstructured proteins" which only adopt a well-defined conformation when bound to other proteins or membrane lipids.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Data are obtained by immunocytochemistry, human iPS derived neurons, fixed in 4% formaldehyde solution. NBP2-25146 diluted 1:1500, followed by staining with Alexa anti chicken–594 (1:1000) secondary antibody. The NB110-55376R was diluted 1:200. All antibodies were diluted in PBS/BSA 3% (w/v) ) Triton X-100 0,3 % (v/v). Images taken by an Arrayscan (Cellomics).