![Western Blot: SYNJ2BP/ARIP2 Antibody [NBP2-94820] - Analysis of extracts of various cell lines, using SYNJ2BP/ARIP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize2/SYNJ2BP-ARIP2-Antibody-Western-Blot-NBP2-94820-img0001.jpg "Western Blot: SYNJ2BP/ARIP2 Antibody [NBP2-94820] - Analysis of extracts of various cell lines, using SYNJ2BP/ARIP2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human SYNJ2BP (NP_060843.2). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SYNJ2BP |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Flow Cytometry: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Analysis of Jurkat cells, using anti-RALBP1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/images2/RALBP1-Antibody-(OTI11B2)-Flow-Cytometry-NBP2-02293-img0001.jpg)
![Western Blot: RALBP1 Antibody (OTI11B2) [NBP2-02293] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.](http://images.novusbio.com/images2/RALBP1-Antibody-(OTI11B2)-Western-Blot-NBP2-02293-img0002.jpg)
![Immunohistochemistry-Paraffin: RALBP1 Antibody (OTI11B2) [NBP2-02293] - Staining of paraffin-embedded breast tissue using anti-RALBP1 mouse monoclonal antibody.](http://images.novusbio.com/images2/RALBP1-Antibody-(OTI11B2)-Immunohistochemistry-Paraffin-NBP2-02293-img0003.jpg)
![Western Blot: REPS1 Antibody [NBP1-86344] - Analysis in control (vector only transfected HEK293T lysate) and REPS1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/images2/REPS1-Antibody-Western-Blot-NBP1-86344-img0004.jpg)
![Immunohistochemistry-Paraffin: REPS1 Antibody [NBP1-86344] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/images2/REPS1-Antibody-Immunohistochemistry-Paraffin-NBP1-86344-img0005.jpg)
![Flow Cytometry: PSD-95 Antibody (6G6-1C9) [NB300-556] - An intracellular stain was performed on HeLa cells with PSD-95 Antibody (6G6-1C9) NB300-556AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/PSD-95-Antibody-(6G6-1C9)-Flow-Cytometry-NB300-556-img0023.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Mixed 3 week old rat cortical cultures (21 DIV) were labelled with anti-PSD95 (1:650) and visualised with anti-mouse Alexa647 (1:2000). The antibody labels small punctae along neuronal processes as expected. This image was submitted via customer Review.](http://images.novusbio.com/images2/PSD-95-Antibody-(6G6-1C9)-Immunocytochemistry-Immunofluorescence-NB300-556-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: PSD-95 Antibody (6G6-1C9) [NB300-556] - Control immunolocalization of plasma membrane AMPA receptor subunits (external epitopes) in hippocampal neuronal cultures. H) Immunolabeling of non-permeabilized neuronal cultures with ani-GluA1 (green). (I) Immunolabeling with the postsynaptic marker anti-PSD95 [66] after permeabilization of neuronal cultures. (J) Merging of (H) and (I) showing postsynaptic labeling of GluA1. Scale bar (valid for all images): 10 um. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0140868), licensed under a CC-BY licence.](http://images.novusbio.com/images2/PSD-95-Antibody-(6G6-1C9)-Immunocytochemistry-Immunofluorescence-NB300-556-img0025.jpg)
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - Tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/ASC-TMS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78977-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - NLRP3 activation was evaluated by visualizing the co-localization of NLRP3 and apoptosis-associated speck-like protein (ASC) using immunocytochemistry (A). PLoS One. 2020 Jun 17;15(6):e0234039. doi: 10.1371/journal.pone.0234039. (Catalog # NBP1-78977AF647)](http://images.novusbio.com/images2/ASC-TMS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78977-img0008.jpg)
![Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Rats were infected by S. pneumoniae or given PBS sham control. 21.5 umoles/kg Mito-Vit-E (MVE) or vehicle was administered orally 30 minutes post-inoculation, and heart tissues were harvested 24 hours later. Heart sections were co-stained with anti-ASC (brown) and haematoxylin (blue). Negative control was stained with secondary antibody alone. Images are representative of a random selection of at least 3 sections of N = 6. PLoS One. 2015 Oct 8;10(10):e0139416. doi: 10.1371/journal.pone.0139416.](http://images.novusbio.com/images2/ASC-TMS1-Antibody-Immunohistochemistry-NBP1-78977-img0009.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for SYNJ2BP/ARIP2 Antibody (NBP2-94820)Discover more about diseases related to SYNJ2BP/ARIP2 Antibody (NBP2-94820).
| Pathways for SYNJ2BP/ARIP2 Antibody (NBP2-94820)View related products by pathway.
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New Players in the Mitophagy Game By Christina Towers, PhD Mitochondrial turn over via the lysosome, otherwise known as mitophagy, involves engulfment of mitochondria into double membrane autophagosomes and subsequent fusion with lysosomes. Much is al... Read full blog post. |
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| Gene Symbol | SYNJ2BP |
![Western Blot: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP1-88039] - Analysis in human cell lines A-549 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/images2/Cytochrome-b5-Outer-Mitochondrial-Membrane-Antibody-Western-Blot-NBP1-88039-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP1-88039] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/images2/Cytochrome-b5-Outer-Mitochondrial-Membrane-Antibody-Immunohistochemistry-Paraffin-NBP1-88039-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cytochrome b5 Outer Mitochondrial Membrane Antibody [NBP1-88039] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/images2/Cytochrome-b5-Outer-Mitochondrial-Membrane-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88039-img0007.jpg)
![Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the alkaline-resistant mitochondrial pellet from cells expressing each BNIP3 phosphomutant. All blots are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY licence.](http://images.novusbio.com/images2/VDAC1-Antibody-Western-Blot-NB100-695-img0006.jpg)
![Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY licence.](http://images.novusbio.com/images2/VDAC1-Antibody-Western-Blot-NB100-695-img0005.jpg)
![Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead).](http://images.novusbio.com/images2/VDAC1-Antibody-Western-Blot-NB100-695-img0004.jpg)
![Flow (Intracellular): Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - An intracellular stain was performed on HEK293 cells with Thymidylate Synthase Antibody [SPM453] NBP2-34441PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin. Image using the Azide and BSA Free format of this antibody.](http://images.novusbio.com/images2/Thymidylate-Synthase-Antibody-(SPM453)-Flow-(Intracellular)-NBP2-34390-img0001.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Thymidylate Synthase Antibody (SPM453).](http://images.novusbio.com/images2/Thymidylate-Synthase-Antibody-(SPM453)-Immunohistochemistry-Paraffin-NBP2-34390-img0003.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (SPM453) [NBP2-34390] - Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Thymidylate Synthase Mouse Monoclonal Antibody (SPM453).](http://images.novusbio.com/images2/Thymidylate-Synthase-Antibody-(SPM453)-Immunohistochemistry-Paraffin-NBP2-34390-img0005.jpg)
![Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images2/Doublecortin-Antibody-Western-Blot-NBP1-72042-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL).](http://images.novusbio.com/images2/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72042-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/images2/Doublecortin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-72042-img0007.jpg)
![Western Blot: VDAC2 Antibody [NBP2-20849] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with VDAC2 antibody [C2C3], C-term diluted at 1:1000.](http://images.novusbio.com/images2/VDAC2-Antibody-Western-Blot-NBP2-20849-img0004.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] - Paraffin-embedded rat kidney. VDAC2 antibody [C2C3], C-term diluted at 1:500.](http://images.novusbio.com/images2/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-20849-img0005.jpg)
![Immunohistochemistry-Paraffin: VDAC2 Antibody [NBP2-20849] - Paraffin-embedded mouse intestine. VDAC2 antibody [C2C3], C-term diluted at 1:500](http://images.novusbio.com/images2/VDAC2-Antibody-Immunohistochemistry-Paraffin-NBP2-20849-img0006.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")