Western Blot: SYK Antibody [NB100-1579] - Samples: Whole cell lysate (5, 15 and 50 ug) from Ramos cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SYK antibody used for WB at 0.1 ug/ml. ...read more
Western Blot: SYK Antibody [NB100-1579] - Detection of Human SYK on HeLa whole cell lysate using NB100-1579. SYK was also immunoprecipitated using rabbit anti-SYK antibody NB100-1578.
Immunoprecipitation: SYK Antibody [NB100-1579] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more
SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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