SYK Antibody

Images

 
Immunohistochemistry: SYK Antibody [NB100-1578] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SYK used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: SYK Antibody [NB100-1578] - Detection of Human SYK by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, ¼ of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NB100-1578
    • Availability
      Product Discontinued

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SYK Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human Spleen Tyrosine Kinase using the numbering given in entry NP_003168.2 (GeneID 6850).
Clonality
Polyclonal
Host
Rabbit
Gene
SYK
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunoprecipitation 1-4 ug/mg of lysate
Application Notes
This antibody is useful for immunoprecipitation. Western blot: Not recommended. Use NB 100-1579, which performs well for WB.
Positive Control
SYK Lysate (NBL1-16637)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SYK Antibody

  • DKFZp313N1010
  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ25043
  • spleen tyrosine kinaseFLJ37489
  • SYK
  • tyrosine-protein kinase SYK

Background

SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional SYK Products

Bioinformatics Tool for SYK Antibody (NB100-1578)

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PTMs for SYK Antibody (NB100-1578)

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Bioinformatics

Gene Symbol SYK
Entrez
Uniprot