SUMO Activating Enzyme E1 (SAE1) Antibody

Images

 
Western Blot: SAE1 Antibody [NBP1-78059] - Coommassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane 1) and purified recombinant GST-SAE1 (lane 2) are ...read more

Order Details


    • Catalog Number
      NBP1-78059
    • Availability
      Product Discontinued

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SUMO Activating Enzyme E1 (SAE1) Antibody Summary

Immunogen
SUMO Activating Enzyme E1 (SAE1) antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 (SAE1).
Specificity
This purified antibody is directed against human SUMO Activating Enzyme E1 protein.
Predicted Species
Mouse (93%), Rat (92%), Canine (96%), Primate (96%), Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SAE1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
Application Notes
This purified antibody has been tested for use in ELISA and western blot. Expect a band at ~60 kDa in size corresponding - SAE1 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

Chimpanzee 96%

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 100 ul deionized water.

Alternate Names for SUMO Activating Enzyme E1 (SAE1) Antibody

  • AOS1
  • AOS1activator of SUMO1
  • FLJ3091
  • HSPC140
  • SAE1
  • SUA1
  • SUA1sentrin/SUMO-activating protein AOS1
  • SUMO Activating Enzyme E1 (SAE1/UBA2)
  • SUMO-1 activating enzyme E1 N subunit
  • SUMO1 activating enzyme subunit 1
  • SUMO-1 activating enzyme subunit 1
  • SUMO-activating enzyme subunit 1
  • UBA2
  • Ubiquitin-like 1-activating enzyme E1A
  • ubiquitin-like protein SUMO-1 activating enzyme
  • UBLE1A

Background

SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the UBL1 conjugation pathway. Proteins conjugated to Ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. This protein can bind with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol SAE1
Entrez
Uniprot