Sucrose Phosphorylase Antibody

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Western Blot: Sucrose Phosphorylase Antibody [NBP2-21666] - Goat anti Sucrose Phosphorylase antibody was used to detect purified Sucrose Phosphorylase under reducing and non-reducing conditions. Reduced samples of ...read more

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Sucrose Phosphorylase Antibody Summary

Description
This antibody was prepared from monospecific antiserum by a delipidation and defibrination.
Immunogen
Sucrose Phosphorylase [E.coli]. Protein accession number YP_852475.1.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Sucrose Phosphorylase [E.coli].
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Delipidation and Defibrination
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:5000
  • ELISA 1:5000-1:20000
  • Immunoprecipitation 1:100
Application Notes
This product has been assayed against 1.0 ug of Sucrose Phosphorylase [E.coli] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #NBP1-73222 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Reactivity Notes

Reactive in E.Coli.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Delipidation and Defibrination
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for Sucrose Phosphorylase Antibody

  • ECK1304
  • JW1302
  • predicted glucosyltransferase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Sucrose Phosphorylase NBP2-21666

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