NB100-65219 recognizes the COOH terminal end of substance P, a short polypeptide neurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. It is known to have a role in several physiologic activities such as pain transmission, the vomiting reflex, salivary secretion and smooth muscle contraction. 5% reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin, somatostatin or beta-endorphin.
Tissue culture supernatant
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunofluorescence. This product does not require antigen retrieval prior to staining of formalin fixed, paraffin embedded sections. This antibody has been used for the localization of substance P in tissue sections by immunofluorescence and PAP staining in nervous systems of experimental animals and man. Please refer to the Cuello references listed below. It has also been successfully applied in archive post-mortem tissues of the human brain, see Mai and Pioro references. Use in Immunohistochemistry whole mount reported in scientific literature (PMID 24728885)
Read 1 Review rated 4 using NB100-65219 in the following applications:
May react with the following species: Bichir, Bovine, Bullfrog, Calanus sp. Cancer sp., Chicken, Chinchilla, Cockroach, Cowbird, Crayfish, Crustacea, Guinea Pig, Homarus sp., Lamprey, Mammals, Mudpuppy, Panilurus sp., Pig, Pigeon, Rabbit, Rat, Sheep, Spadefoot toad, Zebrafish. Amphibia is expected from sequence similiarity. Mouse reactivity reported in scientific literature (PMID: 24728885)
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Substance P (SP) is currently thought to be one of the most important neurotransmitters and neurotransmitters in the brain. It is an undecapeptide belonging to the tachykinin family, which consists of excitatory neuropeptides synthesized in neuronal and glial cells of the human central and peripheral nervous system. Substance P, Neurokinin A, Neuropeptide K, and Neuropeptide gamma are all generated by post-translation cleavage of the precursor Protachykinin-1. Substance P forms the major endogenous ligand for Neurokinin 1 Receptor. The pharmacology of Substance P has been associated with a number of neurological and psychiatric disorders, namely nociception, migraine, asthma, nausea, inflammatory bowel syndrome, urinary incontinence, anxiety and depression. It has also been linked to obesity. This antibody reacts with substance P, a bioactive peptide secreted from neurons both in the gut and central nervous system. Can be used for the localisation of Substance P in cells and tissues.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for Substance P Antibody (NB100-65219)(1)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 2 applications: IHC-Fr, IHC-WhMt.
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