STAT6 Antibody (7D3)

Images

 
Western Blot: STAT6 Antibody (7D3) [NBP1-51624] - Western blot analysis using STAT6 mouse mAb against HEK293 (1), NIH/3T3 (2), MCF-7 (3), Raw246.7 (4) and PC-12 (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: STAT6 Antibody (7D3) [NBP1-51624] - Immunofluorescence analysis of Hela cells using STAT6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
7D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

STAT6 Antibody (7D3) Summary

Immunogen
Purified recombinant fragment of human STAT6 expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
STAT6
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse, Rat, Primate. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for STAT6 Antibody (7D3)

  • D12S1644
  • EC 2.4.1.227
  • EC 2.7.7.6
  • IL-4 Stat
  • IL-4-STAT
  • signal transducer and activator of transcription 6
  • signal transducer and activator of transcription 6, interleukin-4 induced
  • STAT, interleukin4-induced
  • STAT6
  • STAT6B
  • STAT6C
  • transcription factor IL-4 STAT

Background

Janus family tyrosine kinases (Jaks) and signal transducers and activators of transcription (STATs) are involved in mediating gene expression in response to cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the STATs. Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Stat-6 is tyrosine phosphorylated in response to cellular interaction with IL-3 and IL-4 (3,4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STAT6
Entrez